COURSE UNIT TITLE

: BIOCHEMISTRY LABORATORY I

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
KIM 3025 BIOCHEMISTRY LABORATORY I COMPULSORY 0 3 0 3

Offered By

Chemistry

Level of Course Unit

First Cycle Programmes (Bachelor's Degree)

Course Coordinator

PROFESSOR DOCTOR MÜNIRE NALAN DEMIR

Offered to

Chemistry
Chemistry (Evening)

Course Objective

In this lecture the students will learn the basic techniques used in biochemistry laboratory.

Learning Outcomes of the Course Unit

1   1. Make an experimental application to reveal some properties of proteins and amino acids and interpret the results. Students can perform protein isolation from a source and determine the amount of protein in its content.
2   2. Examine the effects of factors such as substrate, enzyme amount, pH and temperature on enzyme activity and interpret the results.
3   3. Make an experimental application to reveal the presence and properties of carbohydrates in biological sources and interpret the results.
4   4. Examine the properties of oils such as solubility and saponification experimentally and interpret the results.
5   5. Will be able to design an experiment for DNA isolation from a biological source.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 GENERAL LABORATORY PRINCIPLES, CHEMICAL HYGENE AND STATISICAL ANALYSIS
2 QUALITATIVE ANALYSIS OF AMINO ACIDS
3 TITRATION CURVES OF AMINO ACIDS
4 TOTAL PROTEIN DETERMINATION
5 PROTEIN ISOLATION FROM DIFFERENT SOURCES
6 AMYLASE ENZYME ACTIVITY VARIATIONS DEPENDENT ON THE ENZYME AND SUBSTRATE CONCENTRATION
7 AMYLASE ENZYME ACTIVITY VARIATIONS DEPENDENT ON PH
8 Midterm Exam
9 INHIBITION OF AMYLASE ENZYME ACTIVITY DEPENDENT ON CADMIUM IONS AND INVESTIGATION OF INHIBITION KINETICS-I
10 INHIBITION OF AMYLASE ENZYME ACTIVITY DEPENDENT ON CADMIUM IONS AND INVESTIGATION OF INHIBITION KINETICS-II
11 IDENTIFICATION OF CARBOHYDRATES AND OSAZONE FORMATION
12 SOLUBILITY OF LIPIDS AND DETERMINATION OF NUMBER OF SAPONIFICATION
13 ISOLATION OF DNA
14 COMPENSATION

Recomended or Required Reading

Textbook(s): Bonner, P.L.R., Hargreavees, A.J., Basic Bioscience Laboratory Techniques, Willey-Blackwell, 2010.
Supplementary Book(s): Glencross H, Ahmed N, Wang Q, Biomedical Science Practice, 978-0-19-953329-9, Oxford University Press.

Planned Learning Activities and Teaching Methods

Interactive expression, Questions and answers, Practice in laboratory

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 FIN FINAL EXAM
4 FCG FINAL COURSE GRADE MTE * 0.30 + ASG * 0.30 + FIN * 0.40
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.30 + ASG * 0.30 + RST * 0.40


*** Resit Exam is Not Administered in Institutions Where Resit is not Applicable.

Further Notes About Assessment Methods

None

Assessment Criteria

Meaningful learning of the concepts of given experimental applications, association to concepts each other, establish cause-effect relationships, to using existed knowledge for evaluation to questions or problems and evaluating of generation ideas are carried out by using the midterm exam, final exam, active participation in the experimental practice. The lecturer informs the student about the assignments and their collection dates within first four weeks. The assignments with signatures are collected within the last two weeks.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

Dokuz Eylul University, Faculty of Science, Department of Chemistry
e-mail: nalan.tuzmen@deu.edu.tr

Office Hours

Wednesday 15.00-17.00

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Tutorials 13 3 39
Preparation for Mid-term Exam 1 2 2
Preparation for Final Exam 1 4 4
Preparing Individual Assignments 11 1 11
Preparation before/after weekly lectures 13 0,5 13
Final 1 2 2
Mid-term 1 2 2
Project Assignment 1 1 1
TOTAL WORKLOAD (hours) 74

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11PO.12PO.13PO.14PO.15PO.16PO.17
LO.155551515555
LO.254551515555
LO.35555515555
LO.455555555
LO.555555555

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

Copyright 2015 DEÜ. BİD

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