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Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS MBG 5031 LABORATORY TECHNIQUES IN MOLECULAR BIOLOGY ELECTIVE 2 2 0 9

Offered By

Molecular Biology and Genetics

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

DOCTOR EVIN IŞCAN

Offered to

Biomedicine and Health Technologies Molecular Biology and Genetics Molecular Biology and Genetics

Course Objective

To introduce the most common laboratory techniques used in the field of molecular biology

Learning Outcomes of the Course Unit

1   to have a solid understanding of the basic laboratory methods used in molecular biology 2   to describe the in silico and wet-lab methods used to predict gene fuction 3   to discuss recombinant DNA technologies 4   to discuss different genome editing technologies 5   to describe the methods used for in vivo, ex vivo and in vitro genome editing 6   to explain the use of molecular biology techniques in functional genomics 7   to be able to perform a literature search and present findings through oral and writter means

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description 1 Overview of Laboratory Techniques Used in Molecular Biology 2 DNA and cDNA Amplification with Traditional PCR and Real-Time PCR (qPCR) 3 Recombinant DNA Technology I 4 Recombinant DNA Technology II 5 Gene Transfer Methods: Transferring DNA to Living Cells and Organisms 6 Assessment of Gene Expression in Mammalian Cells 7 Imaging Techniques 8 MIDTERM 9 Protein Analysis Methods-I 10 Protein Analysis Methods-II 11 CRISPR-Cas Applications 12 Mammalian Cell Culture Techniques-I 13 Mammalian Cell Culture Techniques-II 14 3D Cell Culture: An Overview of Advanced Tools and Techniques 15 FINAL

Recomended or Required Reading

Textbook(s)/References/Materials: Textbook(s): Genomics: Essential Methods, Edited by Mike Starkey abd Ramnath Elaswarapu, Wiley Press, 2010 Methods in Molecular Biology Series Ed.: John M. Walker ISSN: 1064-3745

Planned Learning Activities and Teaching Methods

Theoretical lecture with PowerPoint, literature review/discussion, and assignment presentations by students

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA 1 ASG ASSIGNMENT 2 MTE MIDTERM EXAM 3 FIN FINAL EXAM 4 FCG FINAL COURSE GRADE ASG * 0.30 + MTE * 0.30 + FIN * 0.40 5 RST RESIT 6 FCGR FINAL COURSE GRADE (RESIT) ASG * 0.30 + MTE * 0.30 + RST * 0.40 *** Resit Exam is Not Administered in Institutions Where Resit is not Applicable.

Further Notes About Assessment Methods

None

Assessment Criteria

To be announced.

Language of Instruction

English

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

serif.senturk@deu.edu.tr 02324126514

Office Hours

To be announced.

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours) Lectures 13 3 39 Tutorials 14 1 14 Preparations before/after weekly lectures 13 4 52 Preparation for midterm exam 1 25 25 Preparation for final exam 1 35 35 Preparing assignments 13 4 52 Final 1 2 2 Midterm 1 2 2 TOTAL WORKLOAD (hours) 221

Contribution of Learning Outcomes to Programme Outcomes

PO/LO PO.1 PO.2 PO.3 PO.4 PO.5 PO.6 PO.7 PO.8 LO.1 5 LO.2 5 LO.3 5 LO.4 5 LO.5 5 LO.6 5 LO.7 5