COURSE UNIT TITLE

: GENETICALLY MODIFIED ANIMAL MODELS

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
MBG 6127 GENETICALLY MODIFIED ANIMAL MODELS ELECTIVE 3 0 0 12

Offered By

Molecular Biology and Genetics

Level of Course Unit

Third Cycle Programmes (Doctorate Degree)

Course Coordinator

ASSOCIATE PROFESSOR MUHAMMED KASIM DIRIL

Offered to

Molecular Biology and Genetics
Molecular Biology and Genetics

Course Objective

This course aims to teach students the principles of use and design of advance genicatically modified animal models for functional genomic and human disease research.They will learn to critically read and understand scientific literature and present and discuss scientific research articles in the class. They will learn to do PubMed searches about a selected topic and compile the results in the format of a review article, and how to prepare a sicentific project proposal.

Learning Outcomes of the Course Unit

1   To understand basic animal genetics and various types of inbred strains
2   To learn how to generate transgenic mice
3   To understand biology of embryonal stem cell and culture techniques
4   To design and generate gene knockout/in mice with CRISP technology
5   To understand how to combine modified alleles and design of compound animal models.
6   To acquire understanding of the advanced genetic modified animal models and their use in applied functional genomics research in order to generate new concepts for their use in preclinical research.
7   To gain the ability to critically evaluate the literature on a variety of genetically modified animal models in human disease studies.
8   To improve oral presentation skills
9   To gain the ability to perform online literature searches in repositories such as Pubmed

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Introduction
2 Classical genetics and breeding procedures for laboratory animals
3 Establishment and use of various types of inbred strains
4 Basis of transgenesis by pronuclear DNA injection
5 Biology of Embryonal Stem (ES) cell culture-Paper Discussion
6 Generating gene knockout/in mice with CRISP - Paper Discussion
7 Generating gene knockout/in mice with CRISP - Paper Discussion
8 Midterm Exam
9 Design Use of transgenic gene expression reporter mice - Paper Discussion
10 Use of compound mutant alleles in time and tissue specific gene regulation- Paper Discussion
11 In vivo mutational screens by transgenic mobile DNA elements in animal models for cancer - Paper Discussion
12 Genome editing with CRISPR in hybrid compound ES cells-Paper Discussion
13 Use of advanced genetically modified animals in drug screens by in vivo RNAi expression - Paper Discussion
14 Discussion of an hypothetical project involving advanced genetically modified animal models - Literarature Search
15 Discussion of an hypothetical project involving advanced genetically modified animal models - Literarature Search
16 Final Exam

Recomended or Required Reading

Textbook(s):
Supplementary Book(s):
Hofker and Van Deursen eds. `Transgenic mouse methods and protocols' Methods in Mol. Biology series Human Press ISBN 978-1-60761-973-4
References: various research articles will be used for assignments and case studies

Planned Learning Activities and Teaching Methods

PowerPoint lecture presentations: Lecture subjects will be covered as PowerPoint presentations schematized and simplified for easy visual comprehension.
Research article presentation assignment: Students will be assigned recent research articles on current molecular genetics topics and techniques, present the key points of the article as a short presentation and discuss those with the audience.
Individual assignment: Students will be assigned to do online literature research on a selected topic in molecular genetics and write a paper in the format of a review article. Students will prepare a scientific research project proposal involving the creation and use of relevant transgenic animal models.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 ASG ASSIGNMENT
2 MTE MIDTERM EXAM
3 FIN FINAL EXAM
4 FCG FINAL COURSE GRADE ASG * 0.30 + MTE * 0.30 + FIN * 0.40
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) ASG * 0.30 + MTE * 0.30 + RST * 0.40


*** Resit Exam is Not Administered in Institutions Where Resit is not Applicable.

Further Notes About Assessment Methods

None

Assessment Criteria

To be announced.

Language of Instruction

English

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

+90 232 412 6568

Office Hours

To be announced.

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 14 3 42
Preparations before/after weekly lectures 14 7 98
Preparation for midterm exam 1 25 25
Preparation for final exam 1 30 30
Preparing assignments 5 8 40
Preparing assignments 2 8 16
Preparing presentations 3 15 45
Final 1 2 2
Midterm 1 2 2
TOTAL WORKLOAD (hours) 300

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8
LO.13
LO.23
LO.32
LO.42
LO.52
LO.62
LO.72
LO.82
LO.92

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

Copyright 2015 DEÜ. BİD

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