COURSE UNIT TITLE

: THE BASIC OF TRANLATIONAL LABORATORY METHODS

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
TRO 5017 THE BASIC OF TRANLATIONAL LABORATORY METHODS ELECTIVE 2 2 0 10

Offered By

Translational Oncology

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

ASSOCIATE PROFESSOR GIZEM ÇALIBAŞI KOÇAL

Offered to

Translational Oncology
Dental Biomaterials
Translational Oncology

Course Objective

Cancer Research Laboratory and R & D Laboratories Infrastructure, the sampling in cancer research, laboratory space of phase studies, the diagnostic laboratory techniques, the laboratory techniques that provide for treatment response, tumor models (in vivo in vitro ex vivo, in silico, in toto) Biobanking in cancer research, treatment technologies will be discussed.

Learning Outcomes of the Course Unit

1   Capable of outlined in general laboratory rules and working instructions can express. Capable of apply to basic laboratory analysis methods
2   Be able to obtain and store biological samples in appropriate conditions.
3   Acquire basic knowledge of the basic principles of analysis methods
4   Capable of implementing basic PCR techniques and basic cancer cell culture technic
5   Be able to comment to the results of methods
6   Be able to critically evaluates the published literature in the field of translational oncology and adapt their own applications

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Sample Preparation in Cancer Research
2 Laboratory techniques for diagnosis
3 Tumor genotyping with PCR and Next Generation Sequencing technologies
4 Liquid biopsy, circulating tumor markers and single cell-level analysis
5 Transcript level expression analysis
6 Protein level expression analysis
7 Staining and imaging techniques (immunophistochemistry, immunofluorescence)
8 In vitro tumor models
9 Drug discovery and drug screening studies
10 Ex vivo tumor models- Organoids
11 Microfluidic chip technologies and integration into cancer models
12 In vivo tumor models
13 In silico tumor models
14 Place of laboratory in phase studies
15 Biobanking in cancer research

Recomended or Required Reading

Russell W. Jenkins. Introduction to Ex Vivo Cancer Models. Pages 1-12. In Ex Vivo Engineering of the Tumor Microenvironment. Editor Amir R. Aref David Barbie.
Translational Oncology: An Introduction to Clinical Cancer Research. 2020 by Robert C Bast (Author), Dr Maurie Markman MD (Author), Ernest Hawk (Author).
Lee, M.W., Miljanic, M., Triplett, T. et al. Current methods in translational cancer research.Cancer Metastasis Rev (2020). https://doi.org/10.1007/s10555-020-09931-5
Targeted Therapy in Translational Cancer Research (Translational Oncology) 1st Edition
by Apostolia-Maria Tsimberidou, Razelle Kurzrock (Editor), Kenneth C. Anderson, Robert C. Bast Jr., Maurie Markman, Ernest Hawk
Molecular Biology of the Cell, 6th Edition. Ed. Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter. Garland Publishers
Essential Cell Biology - 4th Edition Bruce Alberts, Dennis Bray, Karen Hopkin, Alexander D Johnson, Julian Lewis, Martin Raff, Keith Roberts, Peter Walter Garland Publishers
The Biology of Cancer, 2nd Edition, Robert A. Weinberg, Garland Publishers
Molecular Biology of the Gene, 7th edition, James Watson, Alexander Gann, Tania Baker, Michael Levine, Stephan Bell, Richard Losick, Stephan Harrison Pearson Cold Spring harbor Laboratory Press.
Principles of Molecular Diagnostics and Personalized Cancer Medicine, Dongfeng Tan, henry Lynch, Wolters Kluwer, Lippincott Williams&Wilkins.
The Handbook of Biomarkers, Kewal Jain, Humana Press
Translational and Experimental Clinical Research, Daniel Schuster, William Powers, Lippincott Williams&Wilkins.
Klinik Araştırmalar Kitabı 2014, Hamdi Akan, Hilal Ilbars, Nurşah Çetinkaya, Bilimsel Tıp Yayınevi
Designing Clinical Research 4th Edition, Steven R Cummings , Warren S Browner , Deborah G Grady , Thomas B Newman , Dr Stephen B Hulley, Lippincott Williams&Wilkins.
Journals
Clinical & translational oncology (1699-048X) in Springer
Translational oncology (1944-7124) in PubMed

Planned Learning Activities and Teaching Methods

Lectures, the topics with classroom activities (presentations and homework assignments) to consolidate and discussion

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 PRF PERFORMANCE
2 FIN FINAL EXAM
3 FCG FINAL COURSE GRADE PRF * 0.40 + FIN*0.60
4 RST RESIT
5 FCGR FINAL COURSE GRADE (RESIT) PRF * 0.40 + FCGR*0.60


*** Resit Exam is Not Administered in Institutions Where Resit is not Applicable.

Further Notes About Assessment Methods

None

Assessment Criteria

1. The learner will clearly define outlined LO1,LO2, LO3, LO4 which will evaluate with exam.
2. The learner will recognize, LO5, LO6 will evaluate with homework, prepared presentations by themselves and exam.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

gizem.calibasi@deu.edu.tr ; 0 232 412 58 35

Office Hours

Monday 13:00-14:00

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 15 4 60
Preparations before/after weekly lectures 15 13 195
Final 1 1 1
TOTAL WORKLOAD (hours) 256

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9
LO.155
LO.255
LO.355
LO.455
LO.555
LO.655

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

Copyright 2015 DEÜ. BİD

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