COURSE UNIT TITLE

: LABORATORY ROTATION IN MOLECULAR MEDICINE I

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
ERS 5006 LABORATORY ROTATION IN MOLECULAR MEDICINE I ELECTIVE 1 4 0 10

Offered By

Molecular Medicine

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR DOCTOR GÜL HÜRAY IŞLEKEL

Offered to

Molecular Medicine

Course Objective

The aim of this course is to allow the students to rotate in different laboratories related with the MSc programme in molecular medicine in a multidisciplinary perpective, to learn about the different research methods and techniques used in research laboratory and to acquire practical knowledge in this area. Basic methods learnrd are as follows: LC-MS/MS, gene clonning, transformation, transfection, siRNA, plazmid izolation, cell culture, Gdna, RNA, protein (total, nuclear) izolation, Agaroz gel electrofhoresis, SDS-PAGE gel electrofhoresis, RT-PCR, real time PCR, western blotting, ımmunopresipitation, ELISA, immunofloresan staining, histone izolation, microarray anaysis, bioinformatics (GEO, GO, ALIGN, EntrezGene data base), cell biology methods

Learning Outcomes of the Course Unit

1   To know about the different laboratories which may be involved in molecular medicine
2   To learn about the different subjects and areas of application in these laboratiores
3   To understand the basic principles of techniques used in these laboratories
4   To compare different methods in terms of principles and applications
5   To be able to apply these methods in ones own reseasrch field
6   To integrate the information acquired and prepare an oral presentation

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Spektrofotometry, Elektrophoresis, cell culture practice
2 Western Blotting, PCR, atomik absorption
3 Spektroflourometry, flourometric free radical measurement
4 Chromatography LC/MS-MS practices
5 TEM Basic principals and practices
6 TEM Basic principals and practices
7 Işık mikroskobunda değerlendirme
8 TEM staining, TEM Image Processing
9 Observation and practices of Molecular biology techniques used in Molecular biology laboratories
10 Method evaluation 1
11 Method evaluation 1
12 Method evaluation 1
13 Method evaluation 1
14 Evaluation of Laboratory Methods

Recomended or Required Reading

1. Biomedical Science Practice-Experimentla and Professional skills (Edited by Hedley Glencross, Nessar Ahmed and Qiuyu Wang). Oxford University press. 2011
2. Tietz Klinik Kimyada Teme Ilkeler (Beşinci Baskıdan Çeviri) Editörler: Catl A. Burtis- Edward R. Ashwood Çeviri Editörü: Diler Aslan. Palme Yayıncılık. 2005
3. Articles on laboratory methods published in SCI or expanded SCI journals.

Planned Learning Activities and Teaching Methods

The course will be organised as rotations in different labs. There will be short presentations from the instructors on the different methods. The students will take notes and prepare a laboratory notebook involving the information learned and processed. In addition, they will be expected to present orally what they have learned.

Assessment Methods

Successful / Unsuccessful


*** Resit Exam is Not Administered in Institutions Where Resit is not Applicable.

Further Notes About Assessment Methods

None

Assessment Criteria

To be announced.

Language of Instruction

English

Course Policies and Rules

The student is expected to read the resources before coming to class. In addition, he/she is expected to contribute actively to discussion.

Contact Details for the Lecturer(s)

Prof Dr. HÜRAY iŞLEKEL
Phone: 412 44 08

Office Hours

Tuesday: 12.30-13:30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 14 4 56
Preparations before/after weekly lectures 14 5 70
Preparation for midterm exam 2 10 20
Preparation for final exam 1 30 30
Preparing presentations 2 10 20
Preparing the laboratory notebook 2 27 54
TOTAL WORKLOAD (hours) 250

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11PO.12PO.13PO.14
LO.15555
LO.25555
LO.3555
LO.4555
LO.5555
LO.655