COURSE UNIT TITLE

: ENZYMOLOGY

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
BIO 5027 ENZYMOLOGY ELECTIVE 2 2 0 9

Offered By

Biochemistry

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR DOCTOR SEZER UYSAL

Offered to

Microbiology
Molecular Medicine
Biochemistry

Course Objective

Enzymes are biological catalyzers forming the basis of metabolism. The aim of this course is to discuss the general properties, catalysis mechanisms, and their roles in metabolism and in the control of metabolism; to analyse enzyme kinetics and types of enzyme inhibition and in addition, evaluate the common enzyme defects using clinical cases.
In the practicals related to the course, the following applications will be conducted: enzyme catalysis and inhibition types, monitoring of enzymatic reactions, and the basics of enzyme activity measurements.
In addition, factors effecting blood enzyme activity levels, physiological roles of some selected enzymes, tissue distributions, and clinical reflections and analysis methods will be studied. The up-to-date use of enzyme tests for diagnosis and monitoring of disease and its principles will be discussed. Finally, it will also be noted that enzymes are used as tools for a variety of laboratory tests.

Learning Outcomes of the Course Unit

1   Understand, at the introductory level, enzymology which is one of the subject areas of biochemistry
2   Have expertise in the theoretical basis of enzymological laboratory practices
3   Analyse at the critical level the basic principles of enzyme action (Ex: types of inhibition, similarities and differences)
4   Synthesis: Synthesize the knowledge on enzymology and apply it to new situations (Ex: Discuss the pharmacokinetics of a drug starting with enzyme kinetics)
5   Present an enzyme with its general, physiological, and clinical properties
6   Demonstrate skills in laboratory work related to enzymes (Ex: Enzyme kinetics, enzyme activity measurement, inhibition).

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Introduction General properties of enzymes Classificaiton of enzymes Small group activity
2 Overview on enzymatic reactions Enzymes as catalyzers Overview on coenzymes Thermodynamics Mecanisms of catalysis Small group activity
3 Enzymatic reactions Enzimatic Activity Michaelis Menten Kinetics Small Group Activity
4 Enzyme Inhibition Use of enzyme inhibition principles in different applications Small Group Activity
5 LABORATORY PRACTICAL: Monitoring of enzymatic reactions spectrophotometrically
6 LABORATORY PRACTICAL: Measurement of Enzyme Activity in biological specimen- End-Point and Kinetic methods
7 Intracellular localisation of enzymes Physiological functions of enzymes Small Group Activity
8 LABORATORY PRACTICAL: Spectophotometric assay of erythrocyte catalase enzyme in UV
9 Regulation of enzyme activity Allostery in enzymes Role of allostery in metabolism Other regulation paths of enzymes
10 Presentations from the students
11 Factors effecting blood enzyme levels Physiological functions and tissue distributions of some selected enzymes Small Group Activity
12 Isoenzymes Use of isoenzymes in clinical enzymology
13 Laboratory assay of isoenzymes: Selected examples
14 Use of enzymes in different applications: Assays of clinical parameters using enzyme-based methods Enzyme-Linked immunosorbent assays Restriction enzymes, PCR, DNA fingerprinting Enzymes as therapeutic agents Small group activity
15 Presentations from the students

Recomended or Required Reading

1. Harper s Illustrated Biochemistry 28th Edition, 2009 , Murray, Bender, Rotham, Kennelly
Rodwell and Weil.McGraw Hill International Edition.pp51-83.
Supplementary textbooks:
2. Textbook of Biochemistry with Clinical Correlations. Seventh Edition 2011, Thomas M. Devlin. John-Wiley& Sons Inc. Publication, USA , pp. 377-418.
3. Textbook of Clinical Chemistry and Molecular Diagnostics. Eds: Burtis CA, Ashwood ER, Bruns DE, 4th edition, 2006, Elsevier Saunders, St Louis.
4. Newsholme, EA and Leech, TR. Functional Biochemistry in Health and Disease 2010, WILEY-BLACKWELL, Oxford, pp 35-68.
5. Bisswanger, H. Practical Enzymology 2011, WILEY-BLACKWELL.
6. Tietz: Klinik Kimyada Temel Ilkeler Beşinci Baskı. (Editörler: Burtis & Ashwood) (Çeviri Editörü: Diler Aslan).PALMY yayıncılık, pp.352-387.

Planned Learning Activities and Teaching Methods

The course will be run in an integrated way-winding theory with case discussion and laboratory practicals. The methods of learning will be interactive.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 PAR PARTICIPATION
4 FIN FINAL EXAM
5 FCGR FINAL COURSE GRADE MTE * 0.30 + ASG * 0.20 +PAR* 0.10 + FIN*0.40
6 RST RESIT
7 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.30 + ASG * 0.20 +PAR* 0.10 + RST*0.40


*** Resit Exam is Not Administered in Institutions Where Resit is not Applicable.

Further Notes About Assessment Methods

None

Assessment Criteria

To be announced.

Language of Instruction

Turkish

Course Policies and Rules

The student is expected to read the resources before coming to class. In addition, he/she is expected to contribute actively to discussion, group-work and practicals. Attending in at least 70 percent of lectures and in at least 80 percent of practicals are mandatory. Plagiarism of any type will result in disciplinary action.

Contact Details for the Lecturer(s)

Prof.Dr.Sezer Uysal:
Phone: 412 44 00- 41 44 01
sezer.uysal@deu.edu.tr

Office Hours

Mondays 12.30-13.30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 15 2 30
Tutorials 15 2 30
Preparations before/after weekly lectures 15 4 60
Preparation for midterm exam 1 20 20
Preparation for final exam 1 40 40
Preparing assignments 1 20 20
Preparing presentations 1 20 20
Other (watching artwork belong to visual arts) 1 5 5
TOTAL WORKLOAD (hours) 225

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8
LO.15
LO.25
LO.3423
LO.423
LO.55
LO.65

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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