COURSE UNIT TITLE

: BASIC METHODS IN MOLECULAR BIOLOGY II

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
TBG 5036 BASIC METHODS IN MOLECULAR BIOLOGY II ELECTIVE 2 4 0 10

Offered By

Medical Biology and Genetics

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR DOCTOR HAKKI OGÜN SERCAN

Offered to

Medical Biology and Genetics
Biochemistry
Molecular Medicine
Laboratory Medicine

Course Objective

To learn basis of recombinat DNA technologies and explain rDNA applications; to get know about gen cloning steps and strategies

Learning Outcomes of the Course Unit

1   to know different types of plasmid vectors using in recombinant DNA technologies
2   to be able to define basic steps of DNA cloning into plasmid vector
3   to know and to be able to explain gen slicing methods
4   to be able to discuss recDNA technologies to understnad gen structure and function
5   to know the basis of trangenic mouse models

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

TBG 5039 - Basic Methods in Molecular Biology I
TBG 5030 - Molecular Genetics

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Course Description
2 Introduction to Recombinant DNA technologies
3 Practical applications of recDNA technologies
4 Properties of plasmids as vector ; how to choose plasmid vector and model for different purposes.
5 Continue..
6 DNA cloning: amplification of spesific region of DNA; RE cutting; ligation; transformation, selection of positive clones
7 Continue
8 Continue
9 +Quiz 1
10 Stabil or transient Gen sliciing and overexpression strategies
11 Inducible gen expression systems
12 +Quiz 2
13 Transgenic animal models
14 Lab. practice
15 Lab. practice
16 Final

Recomended or Required Reading

Current Protocols in Molecular Biology, Wiley, Online ISBN: 9780471142720 , Recombinant DNA: Genes and Genomes . 3rd edition. Yazarlar: James D. Watson, Michael Gilman, Jan A. Witkowski, Mark Zoller. 2007 CSHL press
Molecular Cloning : A laboratory Manual, Fourth ed., CSH press
http://www.protocol-online.org/, http://www.cellbio.com/protocols.html

Planned Learning Activities and Teaching Methods

Course will be performed by a series of presentations which include theoritical knowledge and related-examples with the subjects. It is accepted that students should read about presentation subjects before coming to classroom.
Methods: Interactive presentations

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 FIN FINAL EXAM
4 FCGR FINAL COURSE GRADE MTE * 0.30 + ASG * 0.20 + FIN* 0.50
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.30 + ASG * 0.20 + RST* 0.50


*** Resit Exam is Not Administered in Institutions Where Resit is not Applicable.

Further Notes About Assessment Methods

None

Assessment Criteria

Understanding, learning, analyse, compare, actively contribution

Language of Instruction

Turkish

Course Policies and Rules

Actively contribution in class

Contact Details for the Lecturer(s)

e-posta. esra.erdal@deu.edu.tr Telefon: 412 4609 GSM: + 5324474637

Office Hours

Pazartesi 12.30-13:30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 15 2 30
Tutorials 15 4 60
Preparations before/after weekly lectures 15 3 45
Preparation for midterm exam 5 3 15
Preparation for final exam 1 10 10
Preparation for quiz etc. 3 6 18
Preparing presentations 5 6 30
Other (Lab work, analyse data,...) 6 6 36
Final 1 3 3
Quiz etc. 3 3 9
TOTAL WORKLOAD (hours) 256

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9
LO.1345
LO.2345
LO.3345
LO.4345
LO.5345

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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