COURSE UNIT TITLE

: GENETICS OF MARINE PROKARYOTES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
CDK 5042 GENETICS OF MARINE PROKARYOTES ELECTIVE 3 0 0 7

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR DOCTOR ASLI KAÇAR

Offered to

MARINE LIVING RESOURCES
MARINE LIVING RESOURCES

Course Objective

This course is focused on genetic structures of marine prokaryotes and molecular methods used in the genetic analysis, understanding the mechanisms of which lives in extreme conditions and new genetic resources derived from marine environments

Learning Outcomes of the Course Unit

1   To have knowlege of general properties of marine prokaryotic diversity
2   To understand the genome and prokaryotic chromosome, plasmids and biologic importance of plasmids
3   To learn the molecular methods for investigation of marine procaryotic genomes
4   To have knowlege of the metagenomics of complex microbial community
5   To learn the genetics of marine extremophiles

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Marine Prokaryotic Diversity; Culturable and Non-culturable Bacteria, Proteobacteria, Cyanobacteria
2 Marine Prokaryotic Diversity; Marine Archaea; Methanogens, Extremophiles
3 Genome; Prokaryotic Chromosome, Concept of Operon, Plasmids and Biologic Importance of Plasmids
4 Horizontal Gene Transfer in Marine Microbial Communities; Mutation, Transformation, Transduction
5 Molecular Methods for Investigation of Marine Procaryotic Genomes; PCR, Agarose Gel Electrophoresis, DGGE, FISH
6 Analysis of Total DNA From Procaryotes in Marine Sediments
7 Metagenomics; Metagenomics of Complex Microbial Community, Investigation of New Genes
8 Midterm Exam
9 Genetics of Bacterial Biofilm; Formation of Marine Biofilm, Quorum Sensing Mechanism
10 Genetics of Heavy-Metal Resistant Marine Bacteria; Bioaccumulation, Biosorption, Biotransformation
11 Genetics of Marine Extremophiles; Thermophiles, Psychrophiles, Barophiles, Halophiles
12 Genetics of Bacterial Bioluminescence; Formation of Bioluminescence, GFP and Other Fluorescent Proteins
13 Student Assignments (Discussion)
14 Student Assignments (Discussion)

Recomended or Required Reading

1. Kirchman, L. D. 2008. Microbial Ecology of the Oceans. Second Edition. Wiley-blackwell. A John Wiley and Sons, Inc., Publication. New Jersey, USA, 593 s.
2. Madigan, T, M., Martinko, J. M., Parker, J. 2010. Brock Mikroorganizmaların Biyolojisi. Çeviri Editörü Cumhur Çökmüş. Palme Yayıncılık, Ankara, Turkey, 992s.
3. Klug, S. W., Cummings, M. R. 2003. Concepts of Genetics. 6th edition. Çeviri Editörü Cihan Öner. Palme Yayıncılık. Ankara, Turkey, 816s.
4. Marco, D. 2010. Metagenomics, Theory, Methods and Applications, Caister Academic Press, Norfolk, UK, 212s.
5-Sachse, K., Frey, J. Methods in Molecular Biology: PCR Detection of Microbial Pathogens, Vol:216. Humana Press Inc., Totowa, NJ, 321s.

Planned Learning Activities and Teaching Methods

The course is taught in a lecture, class presentation and discussion format. All class members are expected to attend the lecture and take part in the discussion sessions

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 FIN FINAL EXAM
4 FCG FINAL COURSE GRADE MTE * 0.20 + ASG * 0.40 + FIN * 0.40
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.20 + ASG * 0.40 + RST * 0.40


*** Resit Exam is Not Administered in Institutions Where Resit is not Applicable.

Further Notes About Assessment Methods

Lectures, Homework (Preparing presentations), Referances search, Preparations before/after weekly lectures, Midterm Exam, Final Exam

Assessment Criteria

Midterm Exam (%20), Homework (Preparing presentations) (%40), Final Exam (%40)

Language of Instruction

Turkish

Course Policies and Rules

The students have received lectures on the general microbiology

Contact Details for the Lecturer(s)

Assoc. Dr. Aslı KAÇAR
Institute of Marine Sciences and Technology
Tel: 0090 232 2785565/127
Email: asli.kacar@deu.edu.tr

Office Hours

Friday: 13.30-16.00

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 13 3 39
Preparations before/after weekly lectures 13 4 52
Preparation for midterm exam 1 10 10
Preparation for final exam 1 20 20
Searcing references 5 5 25
Preparing presentations 1 30 30
Midterm 1 2 2
Final 1 2 2
TOTAL WORKLOAD (hours) 180

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8
LO.12344
LO.24324
LO.32
LO.43
LO.53424