COURSE UNIT TITLE

: BASIC CELL AND TISSUE CULTURE TECHNIQUES

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
KIM 4233 BASIC CELL AND TISSUE CULTURE TECHNIQUES ELECTIVE 2 0 0 4

Offered By

Chemistry

Level of Course Unit

First Cycle Programmes (Bachelor's Degree)

Course Coordinator

PROFESSOR DOCTOR MÜNIRE NALAN DEMIR

Offered to

Chemistry (Evening)
Chemistry

Course Objective

It was aimed to give students basic information and principle of cell culture which is one of the current research fields.

Learning Outcomes of the Course Unit

1   Have knowledge about the importance, basic terminology and principle of cell and tissue cultures.
2   Have knowledge and generate ideas as to the space requirements, equipments and materials used in cell culture laboratories.
3   Have knowledge about basic techniques, media, sterilization techniques and the factors caused to contamination and apply them.
4   Have knowledge about microscopic imaging techniques and analyze the images.
5   Have knowledge about subculturing of primary and special cell cultures, cell cloning and scale enlargement in cell culture.
6   Have knowledge about the technological applications of cell culture.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Historical background and introduction to basic terminology of cell and tissue cultures
2 Setting up a cell culture laboratory; space requirements, equipments, culture plasticware and associated small consumable items, maintaining the laboratory
3 The physical environment; temperature, pH, osmolality, CO2 and other gases Surfaces and cell shape; adherent versus nonadherent cells
4 Sterilization; wet heat, dry heat, irradiation, chemical sterilization, filtration
5 Cell culture media; basic components of the cell culture environment, preparation of culture media
6 Standard cell culture techniques; subculturing of adherent and suspension cultures, growth curves and measuring cell growth, freezing and thawing cells
7 Microscopy of living cells; general imaging methodology, imaging of intact cultured cells in flasks, dishes and plates, high-resolution imaging of cells on coverslips, imaging of fluorescently labelled cells, recording of microscopy images, analysis of microscopy images
8 Midterm
9 Contamination; avoid and recognize, method for fluorescent detection of mycoplasma, cross-culture contamination
10 Development and optimization of serum and protein free culture media
11 Primary culture and the establishment of cell lines
12 Specific cell types and their requirements
13 Cell cloning procedures; choice of technique, methods for attached and suspension cells
14 Cell culture for commercial settings; the cell as industrial property, small and large scales

Recomended or Required Reading

John M. Davis, Basic Cell Culture: A Practical Approach, Oxford University Press, (20029.
John M. Davis, Animal Cell Culture: Essential Methods, Wiley-Blackwell, (2011).
P. R. Yadav, Cell Culture, Discovery Publishing House, (2005).
R. Ian Freshney, Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, John Wiley & Sons, (2011).
Jennie P. Mather, Penelope E. Roberts, Introduction to Cell and Tissue Culture: theory and technique, Springer, (1998).

Planned Learning Activities and Teaching Methods

Presentation, Question-answer technique, Homework

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 PAR PARTICIPATION
4 FIN FINAL EXAM
5 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.30 + ASG * 0.20 + PAR * 0.10 + FIN * 0.40
6 RST RESIT
7 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.30 + ASG * 0.20 + PAR * 0.10 + RST * 0.40


*** Resit Exam is Not Administered in Institutions Where Resit is not Applicable.

Further Notes About Assessment Methods

None

Assessment Criteria

Meaningful learning of the basic concepts given in presentations, association of concepts with each other, establishing the cause-result relationships and making comments by using the information available for problems and evaluating idea generation are carried out with mid-term and final exams, active participation to the lecture, homework.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

Dokuz Eylul University, Science Faculty, Chemistry Department
Prof. Dr. Leman Tarhan
e-mail: leman.tarhan@deu.edu.tr

Office Hours

Wednesday 15.00-17.00

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 13 2 26
Preparation before/after weekly lectures 13 1 13
Preparation for Mid-term Exam 1 20 20
Preparation for Final Exam 1 30 30
Preparing Individual Assignments 1 7 7
Final 1 2 2
Mid-term 1 2 2
TOTAL WORKLOAD (hours) 100

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11PO.12PO.13PO.14PO.15PO.16PO.17
LO.145555
LO.255555
LO.3555555
LO.4555555
LO.5555555
LO.6555555

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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