COURSE UNIT TITLE

: BASIC TECHNIQUES IN BIOCHEMISTRY

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
KIM 4207 BASIC TECHNIQUES IN BIOCHEMISTRY ELECTIVE 2 0 0 4

Offered By

Chemistry

Level of Course Unit

First Cycle Programmes (Bachelor's Degree)

Course Coordinator

PROFESSOR DOCTOR HÜLYA AYAR KAYALI

Offered to

Course Objective

In this lecture the students will learn Biological Buffers, Sample Preparation Techniques in Biochemistry, extraction and pre-separation techniques, homogenization / lysis, centrifugation, microfiltration, ultrafiltration, dialysis, precipitation techniques Separation and Purification Techniques of biomolecules, adsorption, gel, ion exchange, affinity, gas and high performance liquid chromatography, electrophoresis applications, issues of Enzymatic Methods of Analysis.

Learning Outcomes of the Course Unit

1   At the end of this course, students able to have knowledge about the techniques used to prepare the sample for in biochemistry researches and generate idea
2   In biochemistry studies can discuss the basis of pre-seperation methods to separate the target molecule from a sample, can generate idea
3   Select of sample preparation methods enzyme activity mesaurment from biological a resource and to have knowledge for application, depend on the circumstances able to scrutinize
4   Have knowledge about advance purification and separation techniques of a biological sample, regarding the applicability of conditions and parameters which need to be considered well-appointed and criticize
5   Determination of a target molecule dependent on biological resource,by making for separetion and further purification, prepare a work program.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Biological Buffers
2 Sample Preparation Techniques in Biochemistry
3 Extraction and Preliminary Separation Techniques
4 Homogenization / lysis, Centrifugation
5 Microfiltration/Ultrafiltration
6 Dialysis, Sedimentation Techniques
7 Separation and Purification Techniques of Biomolecules
8 Mid-term exam
9 Adsorption, Gel Chromatography
10 Ion Exchange Chromatography, Affinity Chromatography
11 Gas Chromatography
12 High-Performance Liquid Chromatography
13 Electrophoresis Applications
14 Methods of Enzymatic Analysis

Recomended or Required Reading

Textbook(s): S.R. Mikkelsen and E. Corton, Bioanalytical Chemistry, Wiley Interscience, (2004).
Supplementary Book(s): K. Wilson, J. Walker, Principles and Techniques of Practical Biochemistry, ISBN: 0 521 65104 2, Cambridge University Press.
D. J. Holme, H. Peck, Analytical Biochemistry, Prentice Hall, (1998).

Planned Learning Activities and Teaching Methods

Presentation, Question-answer technique, Application, Homework

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 FIN FINAL EXAM
4 FCG FINAL COURSE GRADE MTE * 0.30 + ASG * 0.30 + FIN * 0.40
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.30 + ASG * 0.30 + RST * 0.40


*** Resit Exam is Not Administered in Institutions Where Resit is not Applicable.

Further Notes About Assessment Methods

None

Assessment Criteria

Meaningful learning of the basic concepts given in presentations, association of concepts with each other, establishing the cause-result relationships and making comments by using the information available for problems and evaluating idea generation are carried out with mid-term and final exams, active participation to the lecture, homework.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

DEU, Science Faculty, Chemistry Department
Prof. Dr. Leman Tarhan
e-mail: leman.tarhan@deu.edu.tr

Office Hours

Wednesday 15:00 17:00

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 13 2 26
Preparation before/after weekly lectures 13 1 13
Preparation for midterm exam 1 20 20
Preparation for final exam 1 30 30
Preparing Individual Assignments 1 7 7
Midterm 1 2 2
Final 1 2 2
TOTAL WORKLOAD (hours) 100

Contribution of Learning Outcomes to Programme Outcomes

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CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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