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Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS BST 6018 METHODS IN GENE REGULATION ELECTIVE 3 0 0 12

Offered By

Biomedicine and Health Technologies

Level of Course Unit

Third Cycle Programmes (Doctorate Degree)

Course Coordinator

ASSOCIATE PROFESSOR HANI ALOTAIBI

Offered to

Biomedicine and Health Technologies

Course Objective

To gain a general overview of the methods currently used to study gene expression and the regulation of gene expression.

Learning Outcomes of the Course Unit

1   To Understand the theory behind the main techniques used to measure gene expression 2   To Learn reporter assays 3   To learn chromatin related research methods 4   To learn high throughput gene expression methods

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description 1 The central dogma 2 Measuring RNA levels (semi quantitative vs quantitative PCR) 3 Measuring Protein levels 4 Reporter assays for promoter function 5 Reporter assays for enhancer function 6 DNA-Protein interactions 7 Electrophoretic mobility shift assay 8 Midterm 9 Chromatin immunoprecipitation 10 Chromatin conformation measurements 11 DNA methylation 12 RNA interference and silencing 13 High throughput analysis - Microarrays 14 High throughput analysis Next generation sequencing - TF 15 High throughput analysis Next generation sequencing - Histones 16 Final Exam

Recomended or Required Reading

Recent Literature

Planned Learning Activities and Teaching Methods

Discussion based, Problem oriented.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA 1 ASG ASSIGNMENT 2 MTE MIDTERM EXAM 3 FIN FINAL EXAM 4 FCG FINAL COURSE GRADE ASG * 0.30 + MTE * 0.30 + FIN * 0.40 5 RST RESIT 6 FCGR FINAL COURSE GRADE (RESIT) ASG * 0.30 + MTE * 0.30 + RST * 0.40 *** Resit Exam is Not Administered in Institutions Where Resit is not Applicable.

Further Notes About Assessment Methods

None

Assessment Criteria

Theoretical knowledge will be evaluated by exam

Language of Instruction

English

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

hani.alotaibi@deu.edu.tr

Office Hours

To be announced.

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours) Lectures 14 3 42 Preparations before/after weekly lectures 14 8 112 Preparation for final exam 1 30 30 Preparing assignments 2 20 40 Preparing assignments 1 20 20 Preparing presentations 2 15 30 Design Project 1 25 25 Final 1 5 5 TOTAL WORKLOAD (hours) 304

Contribution of Learning Outcomes to Programme Outcomes

PO/LO PO.1 PO.2 PO.3 PO.4 PO.5 PO.6 PO.7 PO.8 LO.1 5 5 5 5 5 LO.2 5 5 5 LO.3 5 5 5 LO.4 5 5 5 5 5 5