Dokuz Eylül University Information Package / Courses Catalog

Description of Individual Course Units

Course Unit Code	Course Unit Title	Type Of Course	D	U	L	ECTS
MOT 6054	LABORATORY APPLICATIONS IN MOLECULAR MEDICINE	COMPULSORY	1	4	0	8

Offered By

Molecular Medicine

Level of Course Unit

Third Cycle Programmes (Doctorate Degree)

Course Coordinator

PROFESSOR DOCTOR GÜL HÜRAY IŞLEKEL

Offered to

Molecular Medicine
Molecular Medicine

Course Objective

The aim of this course is to allow the students to rotate in different laboratories related with the MSc programme in molecular medicine in a multidisciplinary perpective, to learn about the different research methods and techniques used in research laboratory and to acquire practical knowledge in this area. Basic methods learned are as follows: principles of weighing, homogenization techniques, solution preparation, measurement of pH, centrifugation techniques, spectrophotometry, cell culture, gel electrophoresis (agarose, SDS), westernblotting, genomic DNA and total RNA isolation, cDNA synthesis, conventional and real-time PCR, spectrofluorometry, microbiological methods.

Learning Outcomes of the Course Unit

1	To know about the different laboratories which may be involved in molecular medicine
2	To learn about the different subjects and areas of application in these laboratories
3	To understand the basic principles of techniques used in these laboratories
4	To compare different methods in terms of principles and applications
5	To be able to apply these methods in ones own research field
6	To integrate the information acquired and prepare an laboratory notebook

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week	Subject	Description
1	Course Introduction (T) Laboratory Safety and Principles of the laboratory working, General laboratory equipments, Laboratory Preparation, Laboratory notebook preparation (A)
2	Principles of Weighing , SI Units , Solution Preparation (Concentration Calculations) (T) Measurement of pH, Preparation of buffer solutions (A)
3	Principles of homogenization and centrifugation techniques (T, A)
4	Spectrophotometry (T) Glutathione-S-transferase measurement with spectrophotometer, Protein measurement with Biüret, Lowry, Bradford and BCA methods (A)
5	Spectrofluorometry (T) Measurement of naphtalene with spectrofluorometer(A)
6	Electrophoresis (T) Electrophoresis applications, Western Blotting (A)
7	PCR (T) Genomic DNA and Total RNA isolation, cDNA synthesis and conventionall PCR, Real-Time PCR, Agarose gel electrophoresis(A)
8	Cell Culture (T) Cell culture applications (A)
9	Biosafety and research techniques in microbiology (T) Microbiology applications (A)

Recomended or Required Reading

To be announced.

Planned Learning Activities and Teaching Methods

The course will be organised as rotations in different labs. There will be short presentations from the instructors on the different methods. Each course will be evaluated by pre- and post- test. The students will prepare a laboratory notebook involving the information learned and processed.

Assessment Methods

SORTING NUMBER	SHORT CODE	LONG CODE	FORMULA
1	MTE	MIDTERM EXAM
2	QZ	Quiz
3	ASG	ASSIGNMENT
4	FCGR	FINAL COURSE GRADE (RESIT)
5	FCG	FINAL COURSE GRADE	VZ * 0.20 + QZ * 0.15 + ODV * 0.15 + FN*0.50
6	RST	RESIT
7	BBN	BütSonuBaşarıNotu	VZ * 0.20 + QZ * 0.15 + ODV* 0.15 + BUT*0.50

*** Resit Exam is Not Administered in Institutions Where Resit is not Applicable.

Further Notes About Assessment Methods

Each course will be evaluated by pre- and post- test. The students will prepare a laboratory notebook involving the information learned and processed.

Assessment Criteria

Understanding, learning, analyse, compare, application, actively contribution

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

e-mail: huray.islekel@deu.edu.tr
Phone: 412 44 08

Office Hours

Tuesday, 12:30-13:30

Work Placement(s)

None

Workload Calculation

Activities	Number	Time (hours)	Total Work Load (hours)
Lectures	14	1	14
Tutorials	14	4	56
Preparations before/after weekly lectures	14	2	28
Preparation for midterm exam	1	10	10
Preparation for final exam	1	30	30
Preparation for quiz etc.	1	10	10
Preparing presentations	2	10	20
Preparing the laboratory notebook	2	16	32
TOTAL WORKLOAD (hours)			200

Contribution of Learning Outcomes to Programme Outcomes

PO/LO	PO.1	PO.2	PO.3	PO.4	PO.5	PO.6	PO.7	PO.8	PO.9	PO.10	PO.11	PO.12	PO.13	PO.14
LO.1	4	4	5	5	3	3	4	3	3	4	3	3	3	5
LO.2	4	4	5	5	5	5	5	5	5	5	4	5	5	5
LO.3	4	4	5	5	4	4	4	4	4	4	4	5	5	5
LO.4	5	5	5	5	5	5	5	4	5	4	4	5	5	5
LO.5	5	5	5	5	5	5	5	5	5	4	4	5	5	5
LO.6	5	5	5	5	5	5	5	5	5	5	5	4	4	5

COURSE UNIT TITLE

DEGREE PROGRAMMES

Description of Individual Course Units

Offered By

Level of Course Unit

Course Coordinator

Offered to

Course Objective

Learning Outcomes of the Course Unit

Mode of Delivery

Prerequisites and Co-requisites

Recomended Optional Programme Components

Course Contents

Recomended or Required Reading

Planned Learning Activities and Teaching Methods

Assessment Methods

Further Notes About Assessment Methods

Assessment Criteria

Language of Instruction

Course Policies and Rules

Contact Details for the Lecturer(s)

Office Hours

Work Placement(s)

Workload Calculation

Contribution of Learning Outcomes to Programme Outcomes

CONTACT INFORMATION