COURSE UNIT TITLE

Offered By

Biochemistry

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR DOCTOR AYŞE SEMRA KOÇTÜRK

Offered to

Audiology
Laboratory Animal Science
Anatomy
Toxicology
Molecular Medicine
Exercise Physiology
Biochemistry
Biomechanics
Biophysics
Medical Biology and Genetics
Histology and Embryology
Medical Parasitology
Biochemistry
Physiology
Medical Parasitology
Microbiology
Pharmacology
Basic Neuroscience
Molecular Medicine

Course Objective

The aim of this course is the acquisition of knowledge and experience on technics that used in cellular molecular mechanisms research. For this purpose lectures cover the principle of technics that used for isolation of organelles form tissue and cell, the principles of cell culture and application technics and analysis the expression of proteins and genes. Course programmer and applications design in a stream of all technics that provide to monitor intracellular protein and gene expressions with a tissue, a cell line and a primer cell line samples.

Learning Outcomes of the Course Unit

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Recomended or Required Reading

1. Current Protocols in Protein Science. Coligen JE, Dunn BM, Speicher W, Wingfield PT (eds). 2007, John Wiley &Sons Inc.
2. Mitochondria: A Practical Approach. Darley-Usmar UM, Rickwood D&Wilson MT (eds). 1987, IRL Pres.
3. Protein Purification Protocols. 2nd Ed. Cutler P. 2007, Humana Pres.
4. Apoptosis methods and Protocols. Brady HJM (ed) 2005, Humana Pres.
5. Apoptosis, Senescence and Cancer. Gewirtz DA, Halt SE, Grant S. (eds) 2nd ed. 2007, Humana
Press.
6. Cell and Tıssue Culture. Doyle A and Griffiths JB (eds). John Wiley&Sons.
7. Basic Cell Culture Protocols. 3rd Ed. Helgason CH, Miller CL (eds). 2004, Humana Press.
8. Real-Time PCR. Dorak TM (ed), 2007, Taylor&Francis.
9. Basic Methods fort he Biochemical Laboratory. Holtzhouer M. (ed). 2006, Springer.

Planned Learning Activities and Teaching Methods

Laboratory technics will learn with lectures and applications. Experiment design and application skills of learner will evaluate and improve via an assignment.

Assessment Methods

Further Notes About Assessment Methods

None

Assessment Criteria

1. Learner theoretical knowledge about research methods for molecular mechanism will evaluated with mid-term and final exam (PO1,PO2)
2. Learner skills on to reach information and data-base related with the application area, planning of experiment will evaluate via an assignment(PO6,PO7)
3. Learner knowledge on basic laboratory technics that will use to research molecular mechanism will evaluate via all application sessions and an assignment (PO10)

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

semra.kocturk@deu.edu.tr, 4124407

Office Hours

on Tuesday at: 13.30-16.30

Work Placement(s)

None

Workload Calculation

Contribution of Learning Outcomes to Programme Outcomes