COURSE UNIT TITLE

: PROTEIN KINASE C

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
KIM 6114 PROTEIN KINASE C ELECTIVE 3 0 0 7

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR DOCTOR MUSTAFA YAVUZ ERGÜN

Offered to

M.Sc. in Biochemistry
Chemistry
Chemistry
Ph.D in Biochemistry

Course Objective

Within the context of this course, it is aimed to investigate in detail protein phosphorylation reactions and kinases, PKC isotypes and identification, the structure and membrane bindig of PKC C1 and C2 domains, the regülation of PKC by membrane, protein interactions and phosphorylation, the downregulation of PKC, the role of PKC in yeasts as well as the spesific role of atypic PKC.

Learning Outcomes of the Course Unit

1   evaluate protein phosphorylation reactions and protein kinases,
2   know PKC isotypes and identification,
3   reclaim the structure and membrane bindig of PKC C1 and C2 domains, the regülation of PKC by membrane, protein interactions and phosphorylation, and the downregulation of PKC,
4   understand, the role of PKC in yeasts,
5   gain knowledge about the spesific role of atypic PKC.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Introduction 1.1. Protein phosphorylation reactions 1.2. Protein kinases
2 Protein Kinase C 2.1. PKC isoenzymes 2.2. Identification of PKC isoenzymes
3 The Structure, Function and Interaction with Membrane of C1 Domain-1 3.1. The structure of C1 domain and stereospecific ligand identification 3.2. The binding to membrane and intracellular localization
4 The Structure, Function and Interaction with Membrane of C1 Domain-2 4.1. Typic C1 domain of proteins except PKC 4.2. Atypic C1 domain 4.3. C1 region in signaling proteins containing multiple regions
5 The Structural and Functional Properties of C2 domain 5.1. Structure of C2 domain 5.2. Interaction of C2 domain with ligands 5.3. PKC binding to the target in the membrane with C2 domain
6 The Regulation of PKC by Membrane Interactions 6.1. PKC and lipid signaling pathway 6.2. Cofactors: phosphotydilserine, diacyglycerol and Ca+2 6.3. The PKC phosphorylation that determines the intracellular localization
7 The Regulation of PKC by Protein Interactions 7.1. The proteins that binds PKC
8 Midterm Exam
9 The Regulation of PKC with Phosphorylation 1 9.1. The phosphorylation within activation domain 9.2. The phosphorylation within turn motif 9.3. The phosphorylation within hydrofobic domain
10 The Regulation of PKC with Phosphorylation 2 10.1. The role in phosphoinoside 3-kinase pathway 10.2. The regulation of dephosphorylation
11 The Regulation of PKC with Phosphorylation 3 11.1. The role in the cellular function 11.2. PKC regulation by tyrosine phosphorylation
12 PKC downregulation 12.1. Proteolytic mechanism 12.2. The role of downregulation
13 PKC in Yeasts: Mayalarda PKC: Cell Wall Permeability Pathway 13.1- PKC homologs in Yeasts 13.2- Rho GTPases and PKC activation 13.3- Cell Wall Permeability Pathway and cell cycle
14 Presentations

Recomended or Required Reading

Textbook(s): Dekker, L.V. (ed.), 2004, Protein Kinase C, Plemum Publisher: 191 p.
Supplementary Book(s): Kazanietz, M.G. (ed.), 2010, Protein Kinase C in Cancer Signalling and Therapy, Springer, 485 p.

Planned Learning Activities and Teaching Methods

1. Presentation
2. Interactive narrative
3. Question-answer technique
4. Home work

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 ASG ASSIGNMENT
2 MTE MIDTERM EXAM
3 FIN FINAL EXAM
4 FCG FINAL COURSE GRADE ASG * 0.30 + MTE * 0.30 + FIN * 0.40
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) ASG * 0.30 + MTE * 0.30 + RST * 0.40


*** Resit Exam is Not Administered in Institutions Where Resit is not Applicable.

Further Notes About Assessment Methods

None

Assessment Criteria

Meaningful learning of the basic concepts given in presentations, association of concepts with each other, establishing the cause-result relationships and making comments by using the information available for problems and evaluating idea generation are carried out with mid-term and final exams, active participation to the lecture, homework.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

hulya.kayali@deu.edu.tr

Office Hours

Wednesday 13.30-15.30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 13 3 39
Preparations before/after weekly lectures 13 3 39
Preparation for midterm exam 1 10 10
Preparation for final exam 1 20 20
Preparing assignments 2 15 30
Preparing presentations 2 15 30
Final 1 2 2
Midterm 1 2 2
TOTAL WORKLOAD (hours) 172

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11
LO.15555555
LO.25555555
LO.35555555
LO.45555555
LO.555555555

CONTACT INFORMATION
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Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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