COURSE UNIT TITLE

: LABORATORY METHODS USED IN MOLECULAR PATHOLOGY

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
MPT 6009 LABORATORY METHODS USED IN MOLECULAR PATHOLOGY ELECTIVE 3 2 0 12

Offered By

Molecular Pathology

Level of Course Unit

Third Cycle Programmes (Doctorate Degree)

Course Coordinator

ASSOCIATE PROFESSOR ANIL AYSAL AĞALAR

Offered to

Molecular Pathology

Course Objective

The aim of this course is to learn macroscopic sampling, tissue processing and sectioning, tissue storage, histochemical and immunohistochemical staining methods, DNA and RNA isolation, hybridization, polymerase chain reaction as well as sequencing procedures; and therefore to identify application methods and areas of molecular pathology.

Learning Outcomes of the Course Unit

1   Students will be able to discuss and practice storage of cells and tissues, and fixation characteristics for molecular pathologic examination
2   Students will be able to discuss and practice association of macroscopic evaluation and sampling methods with molecular pathologic examination
3   Students will be able to discuss and practice association of tissue processing, paraffin-embedding, sectioning and histochemical staining methods with molecular pathologic examination
4   Students will be able to discuss and practice principles of immunohistochemical staining methods
5   Students will be able to discuss and practice DNA and RNA isolation, polymerase chain reaction, gel electrophoresis and sequencing procedures
6   Students will be able to discuss and practice different types and application methods of in situ hybridization
7   Students will be able to discuss and practice methods and application areas of microarray
8   Students will be able to discuss and practice topics about safety of both molecular pathology laboratory employees and diagnosis

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Security criteria of molecular pathology laboratory in terms of employee and patient diagnosis
2 Association of macroscopic evaluation and sampling methods with molecular pathologic examination
3 Association of tissue processing, paraffin-embedding and sectioning with molecular pathologic examination
4 Histochemical staining methods
5 Mechanism of immunohistochemical /immunofluorescent staining procedures
6 Application methods of immunohistochemical /immunofluorescent biomarkers in pathologic diagnosis
7 RNA and DNA isolation
8 Polymerase chain reaction and its application areas in molecular pathology
9 Sequencing procedures I
10 Sequencing procedures II
11 Fluorescent in situ hybridization methods
12 Non-fluorescent in situ hybridization methods
13 Microarray methods
14 Methods of archiving and molecular pathologic evaluation
15 Cell/tissue selection for molecular pathology

Recomended or Required Reading

Primary textbook: Molecular Pathology (Turkish) 2017 Bartlett JMS, Shaaban A, Schmitt F.
Supplementary book(s): Robbins Pathological Basis of Diseases 2015 9th ed.
Diagnostic Pathology, Molecular Oncology Vasef MA, Auerbach A. 2016

Planned Learning Activities and Teaching Methods

1- Theoretical lecture presentations
2- Homework preperation and presentations
3- Laboratory studies

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 LAB LABORATORY
4 FIN FINAL EXAM
5 FCGR FINAL COURSE GRADE MTE * 0.20 + ASG * 0.10 + LAB* 0.20 + FIN*0.50
6 RST RESIT
7 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.20 + ASG * 0.10 + LAB* 0.20 + RST*0.50


Further Notes About Assessment Methods

None

Assessment Criteria

Learning subjects of the lectures will be evaluated by classical exams and homework assignments /presentations.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

00902324123401, 0090232413408
sulen.sarioglu@deu.edu.tr
sulensari@gmail.com

Office Hours

Tuesday 11.00-12:00

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 15 3 45
Labratory 15 2 30
Preparations before/after weekly lectures 15 4 60
Preparing presentations 1 30 30
Preparation for final exam 1 60 60
Preparation for midterm exam 1 24 24
Preparing assignments 1 45 45
Final 1 3 3
Midterm 1 3 3
TOTAL WORKLOAD (hours) 300

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11
LO.155554
LO.255554
LO.355554
LO.4555544
LO.5555544
LO.6555544
LO.755554
LO.855554

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

Copyright 2015 DEÜ. BİD

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