COURSE UNIT TITLE

: BASIC TECHNIQUES IN BIOCHEMISTRY

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
KIM 4249 BASIC TECHNIQUES IN BIOCHEMISTRY ELECTIVE 2 0 0 3

Offered By

Chemistry

Level of Course Unit

First Cycle Programmes (Bachelor's Degree)

Course Coordinator

PROFESSOR DOCTOR RAZIYE ÖZTÜRK ÜREK

Offered to

Chemistry

Course Objective

In this lecture the students will learn Biological Buffers, Sample Preparation Techniques in Biochemistry, extraction and pre-separation techniques, homogenization / lysis, centrifugation, microfiltration, ultrafiltration, dialysis, precipitation techniques Separation and Purification Techniques of biomolecules, adsorption, gel, ion exchange, affinity, gas and high performance liquid chromatography, electrophoresis applications, issues of Enzymatic Methods of Analysis.

Learning Outcomes of the Course Unit

1   At the end of this course, students able to have knowledge about the techniques used to prepare the sample for in biochemistry researches and generate idea,
2   In biochemistry studies can discuss the basis of pre-seperation methods to separate the target molecule from a sample, can generate idea,
3   Select of sample preparation methods enzyme activity mesaurment from biological a resource and have knowledge for application, evaluate results depend on the circumstances,
4   have knowledge about advance purification and separation techniques of a biological sample, regarding the applicability of conditions and parameters which need to be considered well-appointed and criticize,
5   determinate of a target molecule dependent on biological resource,by making for separetion and further purification, prepare a work program.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Biological Buffers
2 Sample Preparation Techniques in Biochemistry
3 Extraction and Preliminary Separation Techniques
4 Homogenization / lysis, Centrifugation
5 Microfiltration/Ultrafiltration
6 Dialysis, Sedimentation Techniques
7 Separation and Purification Techniques of Biomolecules
8 Purification table
9 Adsorption, Gel Chromatography
10 Ion Exchange Chromatography, Affinity Chromatography
11 Gas Chromatography
12 High-Performance Liquid Chromatography
13 Electrophoresis Applications
14 Methods of Enzymatic Analysis and Student Presentations

Recomended or Required Reading


Ana kaynak: Glencross H, Ahmed N, Wang Q, Biomedical Science Practice, 978-0-19-8831228, Oxford University Press, 2023.
S.R. Mikkelsen and E. Corton, Bioanalytical Chemistry, Wiley Interscience, 2004.
Yardımcı kaynaklar: K. Wilson, J. Walker, Principles and Techniques of Practical Biochemistry, ISBN: 0 521 65104 2, Cambridge University Press, 2000.
D. J. Holme, H. Peck, Analytical Biochemistry, Prentice Hall, 1998.

Planned Learning Activities and Teaching Methods

Presentation
Questions and answers
Homework

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 FIN FINAL EXAM
4 FCG FINAL COURSE GRADE MTE * 0.30 + ASG * 0.30 + FIN * 0.40
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.30 + ASG * 0.30 + RST * 0.40


Further Notes About Assessment Methods

None

Assessment Criteria

To be announced.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

DEU Science Fac., Chemistry Dep. Biochemistry Division, Buca-IZMIR
raziye.urek@deu.edu.tr

Office Hours

Wednesday 16:00-17:00

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 14 2 28
Preparations before/after weekly lectures 14 1 14
Preparation for midterm exam 1 10 10
Preparation for final exam 1 15 15
Preparing assignments 1 5 5
Final 1 2 2
Midterm 1 1 1
TOTAL WORKLOAD (hours) 75

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11PO.12PO.13PO.14PO.15PO.16PO.17
LO.1555555555
LO.2555555555
LO.3555555555
LO.4555555555
LO.55555355555

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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