COURSE UNIT TITLE

: BIOCHEMISTRY LABORATORY II

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
ELECTIVE

Offered By

Chemistry

Level of Course Unit

First Cycle Programmes (Bachelor's Degree)

Course Coordinator

PROFESSOR DOCTOR RAZIYE ÖZTÜRK ÜREK

Offered to

Chemistry

Course Objective

Within the scope of this course; It is aimed that students learn the basic techniques in biochemistry laboratory.

Learning Outcomes of the Course Unit

1   They can; determine Pyruvate and vitamin C analyzes and discuss the results.
2   Tcharacterize the enzymatic synthesis and characterization of glucose-1-phosphate from starch and discuss the results.
3   determine lipid-peroxidation and interpret the results.
4   plan, implement and interpret the experimental stages required for cholesterol, proline, urea, and inorganic phosphorus analyzes.
5   plan, apply and interpret the experimental stages required for microbial production with fermentation technology.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

KIM 3037 - Biochemistry Laboratory I

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 General Principles In Laboratory, Chemical Hygene, Statistical Analysis
2 Pyruvate Determination
3 Enzymatic Glucose-1 Phosphate Formation from Starch I
4 Enzymatic Glucose-1 Phosphate Formation from Starch II
5 Enzymatic Glucose-1 Phosphate Formation from Starch III
6 Lipid Peroxidation
7 Cholesterol Determination
8 Ascorbic Acid Determination
9 Spectrophotometric Determination of the amount of Proline in Plants
10 Urea and Inorganic Phosphorus Determination
11 Microbial Protein Production and Purification via Fermentation Technology I
12 Microbial Protein Production and Purification via Fermentation Technology II
13 Experimental Design
14 Compensation

Recomended or Required Reading

Textbook(s):
Nessar Ahmed, Biomedical Science Practice, 3rd Edition, 978-0198831228, Oxford University Press, 2023.
Clark, J.m., Switzer R.L. Experimental Biochemistry, 2.nd Edition, W. H. Freeman and Company, 0-7167-0179-0


Supplementary Book(s):
Bonner, P.L.R., Hargreavees, A.J., Basic Bioscience Laboratory Techniques, Willey-Blackwell, 2010.

Planned Learning Activities and Teaching Methods

Interactive expression, Question and answer technique, Experimental application and homework.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 FIN FINAL EXAM
4 FCG FINAL COURSE GRADE MTE * 0.30 + ASG * 0.30 + FIN * 0.40
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.30 + ASG * 0.30 + RST * 0.40


Further Notes About Assessment Methods

None

Assessment Criteria

Meaningful learning of the basic concepts given in presentations, association of concepts with each other, establishing the cause-result relationships and making comments by using the information available for problems and evaluating idea generation are carried out with mid-term and final exams, homeworks.

Language of Instruction

Turkish

Course Policies and Rules

it will be announced.

Contact Details for the Lecturer(s)

DEU Sci Fac Chemistry Dept. Biochemistry Division Buca-Izmir
raziye.urek@deu.edu.tr

Office Hours

Friday 12:00-13:00

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Labratory 14 3 42
Preparations before/after weekly lectures 13 2 26
Preparation for midterm exam 1 10 10
Preparation for final exam 1 15 15
Preparation for quiz etc. 0 0 0
Preparing assignments 8 1 8
Final 1 2 2
Midterm 1 1 1
TOTAL WORKLOAD (hours) 104

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11PO.12PO.13PO.14PO.15PO.16PO.17
LO.14555123345555
LO.235551345555
LO.3355531345555
LO.435554345555
LO.535552345555

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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