COURSE UNIT TITLE

: BIOCHEMISTRY LABORATORY II

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
KIM 3038 BIOCHEMISTRY LABORATORY II COMPULSORY 0 0 3 4

Offered By

Chemistry

Level of Course Unit

First Cycle Programmes (Bachelor's Degree)

Course Coordinator

PROFESSOR DOCTOR RAZIYE ÖZTÜRK ÜREK

Offered to

Chemistry

Course Objective

Within the scope of this course; It is aimed that students learn the basic techniques in biochemistry laboratory.

Learning Outcomes of the Course Unit

1   They can; determine Pyruvate and vitamin C analyzes and discuss the results.
2   Tcharacterize the enzymatic synthesis and characterization of glucose-1-phosphate from starch and discuss the results.
3   determine lipid-peroxidation and interpret the results.
4   plan, implement and interpret the experimental stages required for cholesterol, proline, urea, and inorganic phosphorus analyzes.
5   plan, apply and interpret the experimental stages required for microbial production with fermentation technology.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

KIM 3037 - Biochemistry Laboratory I

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 General Principles In Laboratory, Chemical Hygene, Statistical Analysis
2 Pyruvate Determination
3 Enzymatic Glucose-1 Phosphate Formation from Starch I
4 Enzymatic Glucose-1 Phosphate Formation from Starch II
5 Enzymatic Glucose-1 Phosphate Formation from Starch III
6 Lipid Peroxidation
7 Cholesterol Determination
8 Ascorbic Acid Determination
9 Spectrophotometric Determination of the amount of Proline in Plants
10 Urea and Inorganic Phosphorus Determination
11 Microbial Protein Production and Purification via Fermentation Technology I
12 Microbial Protein Production and Purification via Fermentation Technology II
13 Experimental Design
14 Compensation

Recomended or Required Reading

Textbook(s):
Nessar Ahmed, Biomedical Science Practice, 3rd Edition, 978-0198831228, Oxford University Press, 2023.
Clark, J.m., Switzer R.L. Experimental Biochemistry, 2.nd Edition, W. H. Freeman and Company, 0-7167-0179-0


Supplementary Book(s):
Bonner, P.L.R., Hargreavees, A.J., Basic Bioscience Laboratory Techniques, Willey-Blackwell, 2010.

Planned Learning Activities and Teaching Methods

Interactive expression, Question and answer technique, Experimental application and homework.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 FIN FINAL EXAM
4 FCG FINAL COURSE GRADE MTE * 0.30 + ASG * 0.30 + FIN * 0.40
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.30 + ASG * 0.30 + RST * 0.40


Further Notes About Assessment Methods

None

Assessment Criteria

Meaningful learning of the basic concepts given in presentations, association of concepts with each other, establishing the cause-result relationships and making comments by using the information available for problems and evaluating idea generation are carried out with mid-term and final exams, homeworks.

Language of Instruction

Turkish

Course Policies and Rules

it will be announced.

Contact Details for the Lecturer(s)

DEU Sci Fac Chemistry Dept. Biochemistry Division Buca-Izmir
raziye.urek@deu.edu.tr

Office Hours

Friday 12:00-13:00

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Labratory 14 3 42
Preparations before/after weekly lectures 13 2 26
Preparation for midterm exam 1 10 10
Preparation for final exam 1 15 15
Preparation for quiz etc. 0 0 0
Preparing assignments 8 1 8
Final 1 2 2
Midterm 1 1 1
TOTAL WORKLOAD (hours) 104

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11PO.12PO.13PO.14PO.15PO.16PO.17
LO.14555123345555
LO.235551345555
LO.3355531345555
LO.435554345555
LO.535552345555

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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