COURSE UNIT TITLE

: RECOMBINANT DNA TECHNOLOGIES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
MBG 6124 RECOMBINANT DNA TECHNOLOGIES ELECTIVE 2 2 0 12

Offered By

Molecular Biology and Genetics

Level of Course Unit

Third Cycle Programmes (Doctorate Degree)

Course Coordinator

ASSOCIATE PROFESSOR HANI ALOTAIBI

Offered to

Molecular Biology and Genetics
Molecular Biology and Genetics

Course Objective

This course will provide an understanding of the science and techniques of recombinant DNA molecular biotechnology, teach the students the past, current and future of molecular biotechnology. Basics techniques and applications used in molecular biotechnology will be thaugt in this course.

Learning Outcomes of the Course Unit

1   Describe the different techniques used in recombinant DNA technology
2   Determine the technique(s) suitable for solving a specific experimental problem.
3   Delineate the steps used in gene manipulation, gene expression techniques, and recombinant protein development, and relate these processes to current commercial and research applications.
4   Describe the common methods and applications of biotechnology with regards to microorganisms, plants and animals.
5   Acknowledge both sides of the ethical implications of biotechnology.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 History of molecular biotechnology
2 Principles of Recombinant DNA technology
3 Chemical Synthesis, Sequencing, and Amplification of DNA
4 Molecular Tools for Gene Cloning
5 Vectors in Recombinant DNA technology
6 Manipulation of gene expression in prokaryotes
7 Heterologous Protein Production in Eukaryotic Cells
8 Midterm
9 Common Methods and Applications of Animal, Human and Medical Biotechnology
10 Common Methods and Applications of Microbial and Environmental Biotechnology
11 The Development of Pharmaceutical Products
12 Therapeutic Biologicals
13 Discussion of Assigned Journal Articles
14 Discussion of Assigned Journal Articles
15 Discussion of Assigned Journal Articles
16 Final Exam

Recomended or Required Reading

1- T. A. Brown, Gene Cloning and DNA Analysis: An Introduction, 7th Edition, Wiley, 2016
2- Bernard R. Glick, Jack J. Pasternak, Cheryl L. Patten, Molecular Biotechnology: Principles and Applications of Recombinant DNA, 4th Edition, ASM Press, 2010

Planned Learning Activities and Teaching Methods

1. Presentation
2. Interactive narrative
3. Question-answer technique
4. Homework

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 ASG ASSIGNMENT
2 MTE MIDTERM EXAM
3 FIN FINAL EXAM
4 FCG FINAL COURSE GRADE ASG * 0.30 + MTE * 0.30 + FIN * 0.40
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) ASG * 0.30 + MTE * 0.30 + RST * 0.40


Further Notes About Assessment Methods

None

Assessment Criteria

Meaningful learning of the basic concepts given in presentations, association of concepts with each other, establishing the cause-result relationships and making comments by using the information available for problems and evaluating idea generation are carried out with mid-term and final exams, active participation to the lecture, homework.

Language of Instruction

English

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

02324126514

serif.senturk@deu.edu.tr

Office Hours

Wednesday 15.30-17.00

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 14 3 42
Preparations before/after weekly lectures 14 7 98
Preparation for midterm exam 1 25 25
Preparation for final exam 1 30 30
Preparing assignments 5 8 40
Preparing assignments 2 8 16
Preparing presentations 3 15 45
Final 1 2 2
Midterm 1 2 2
TOTAL WORKLOAD (hours) 300

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8
LO.12
LO.22
LO.32
LO.42
LO.52