COURSE UNIT TITLE

: MOLECULAR BIOLOGY TECHNIQUES II

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
BIY 4134 MOLECULAR BIOLOGY TECHNIQUES II ELECTIVE 3 0 0 5

Offered By

Biology

Level of Course Unit

First Cycle Programmes (Bachelor's Degree)

Course Coordinator

PROFESSOR DOCTOR ARZU GÖRMEZ

Offered to

Biology

Course Objective

The aim of this course is to teach the current techniques used in molecular biology.

Learning Outcomes of the Course Unit

1   will be able to explain the isolation and purification methods of proteins, which are the basic molecules of molecular biology.
2   will be able to explain and compare the analysis methods of enzymes and proteins.
3   will be able to give information about the current molecular methods on enzymes and proteins.
4   will be able to express transcriptomic studies with molecular methods carried out on RNA molecules.
5   will be able to comprehend the strengths and weaknesses of different experimental approaches used in molecular biology.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Molecular Methods carried out by RNA
2 Transcriptomic Studies
3 Isolation and Analysis of Proteins
4 Purification of Proteins
5 Chromatographic and Electrophoretic Methods Used in Purification of Proteins
6 Multidimensional Techniques in Proteomics Studies
7 Protein Determination Methods; Spectral Methods Used in the Determination of Proteins
8 Protein Analysis Methods (Aminoacid Analysis, Sequencing, and Characterization)
9 Protein Analysis Methods (Characterization)
10 Mass Spectroscopy (MS) Methods Used in the Analysis of Proteins
11 Protein Fingerprint Analysis and Microarrays
12 Serological Methods (ELISA)
13 Enzymatic Analysis and Enzyme Activity Tests
14 Protein Hybridization (Western Blot and Immunodetection) An Overview of Methods Used in Molecular Biology

Recomended or Required Reading

1. Gormez, A. 2024. Unpublished Methods Used in Molecular Biology II Lecture Notes. Izmir.
2. Temizkan, G., Arda, N., 2021. Basic and Advanced Molecular Biology Methods Genomic and Proteomic Analysis, 2nd Edition Nobel Medicine, Istanbul.

Planned Learning Activities and Teaching Methods

The course is taught in a lecture, class presentation, and discussion format. All class members are expected to attend the lecture and participate in the discussion sessions.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 VZ Vize
2 FN Final
3 BNS BNS VZ * 0.40 + FN * 0.60
4 BUT Bütünleme Notu
5 BBN Bütünleme Sonu Başarı Notu VZ * 0.40 + BUT * 0.60


Further Notes About Assessment Methods

None

Assessment Criteria

The student is evaluated with a midterm exam and a final exam.

Language of Instruction

Turkish

Course Policies and Rules

It is the student's responsibility to attend 70% of the courses during the semester. Students are required to be present during the lesson and on the days when the presentation is required. Regarding unethical behaviors that may occur in classes and exams, action is taken within the framework of the relevant regulation.

Contact Details for the Lecturer(s)

arzu.gormez@deu.edu.tr

Office Hours

It will be announced.

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 14 3 42
Preparations before/after weekly lectures 13 3 39
Preparation for midterm exam 1 15 15
Preparation for final exam 1 20 20
Final 1 2 2
Midterm 1 1 1
TOTAL WORKLOAD (hours) 119

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11PO.12PO.13PO.14
LO.15554434554
LO.25554444554
LO.35554444554
LO.45554444554
LO.5555444445

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

Copyright 2015 DEÜ. BİD

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