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Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS TRO 6025 PROTEOMICS ELECTIVE 5 0 0 8

Offered By

Translational Oncology

Level of Course Unit

Third Cycle Programmes (Doctorate Degree)

Course Coordinator

PROFESSOR DOCTOR YASEMIN BAŞBINAR

Offered to

Canser Epidemiology Doctorate Translational Oncology

Course Objective

The purpose of this course is to introduce proteomics field, Understand the importance of proteomic analysis and proteomic techniques to provide knowledge about.

Learning Outcomes of the Course Unit

1   Knowledgeable about cancer proteomics. 2   Be able to critically evaluate the published literature in the field of cancer proteomics methods. 3   Be able to know bioinformatics on proteomics 4   Be able to understand the diagnostic importance of cancer proteomics

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description 1 Course description and general concepts Matrix assisted laser desorption ionization-time off flight (Maldi-tof)-MS) 2 Proteom discription and significance Determination of post-translational modifications, Amino acid sequencing: Edman degredation 3 Basic structure and functions of proteins Foruer Transform Spectroscopy 4 2D-gel electrophoresis: SDS-PAGE and isoelectric focusing (IEF) Antibody based proteomics 5 Visualtization of proteins Bioinformatics on proteomics 6 Protein enrichment methodology 7 Sample preparation in MS 8 In-gel digestion 9 Mass spectrometric (MS) analysis of proteins 10 Electrospray ionization (ESI)-MS , Tandem MS

Recomended or Required Reading

To be announced.

Planned Learning Activities and Teaching Methods

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA 1 MTE MIDTERM EXAM 2 PRF PERFORMANCE 3 FIN FINAL EXAM 4 FCG FINAL COURSE GRADE MTE * 0.20 +PRF * 0.20 + FIN* 0.60 5 RST RESIT 6 FCGR FINAL COURSE GRADE MTE * 0.20 +PRF * 0.20 +RST* 0.60

Further Notes About Assessment Methods

None

Assessment Criteria

To be announced.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

To be announced.

Office Hours

To be announced.

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours) Lectures 15 5 75 Preparation for midterm exam 5 4 20 Preparation for final exam 4 4 16 Preparing assignments 5 6 30 Preparing presentations 5 6 30 Preparations before/after weekly lectures 15 2 30 Final 1 1 1 Midterm 1 1 1 TOTAL WORKLOAD (hours) 203

Contribution of Learning Outcomes to Programme Outcomes

PO/LO PO.1 PO.2 PO.3 PO.4 PO.5 PO.6 PO.7 PO.8 PO.9 PO.10 PO.11 PO.12 LO.1 4 LO.2 4 4 4 4 LO.3 4 LO.4 4 4 4