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Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS TLT 2258 MOLECULAR BIOLOGIC METHODS COMPULSORY 2 0 0 3

Offered By

Medical Laboratory Techniques

Level of Course Unit

Short Cycle Programmes (Associate's Degree)

Course Coordinator

ASSOCIATE PROFESSOR NESLIHAN TOYRAN ALOTAIBI

Offered to

Medical Laboratory Techniques

Course Objective

The purpose of this course, students of molecular biology and genetics laboratories learning tools and techniques commonly used, for what purpose the tests, and to provide an understanding of how the mechanisms.

Learning Outcomes of the Course Unit

1   Tools and techniques commonly used in molecular biology laboratory have been learned. 2   Gain knowledge and experience about the DNA Isolation 3   Polymerase chain reaction (PCR) and gene duplication of information and experience on win 4   gain knowledge and experience about RNA Isolation 5   gain experience about Nucleic acids from agarose and acrylamide gels and information monitoring

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description 1 Introduction and general promotion of laboratory instruments Doç. Dr. Neslihan Toyran Alotaibi 2 General Laboratory Procedures, Equipment Use and Security precautions Doç. Dr. Neslihan Toyran Alotaibi 3 DNA isolation Doç. Dr. Neslihan Toyran Alotaibi 4 Polymerase chain reaction (PCR) and gene duplication Doç. Dr. Neslihan Toyran Alotaibi 5 Mixi prepare PZR and mix amplification to ensure that the distribution Doç. Dr. Neslihan Toyran Alotaibi 6 Multiplex PCR Doç. Dr. Neslihan Toyran Alotaibi 7 RNA isolations Doç. Dr. Neslihan Toyran Alotaibi 8 Preparation of agarose and acrylamide gel Doç. Dr. Neslihan Toyran Alotaibi 9 Agarose and acrylamide monitoring of nucleic acids jellies Doç. Dr. Neslihan Toyran Alotaibi 10 RFLP Doç. Dr. Neslihan Toyran Alotaibi 11 RFLP mixi, preparing to make the distribution of Mix and start cutting Doç. Dr. Neslihan Toyran Alotaibi 12 Install the product gel and nitrocellulose filter transfer Doç. Dr. Neslihan Toyran Alotaibi

Recomended or Required Reading

presentations

Planned Learning Activities and Teaching Methods

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA 1 AS ArasınavTeo 2 FCGR FINAL COURSE GRADE 3 FCG FINAL COURSE GRADE AS * 0.50 + FN * 0.50 4 RST RESIT 5 FCGR FINAL COURSE GRADE (RESIT) AS * 0.50 + BUT * 0.50

Further Notes About Assessment Methods

None

Assessment Criteria

To be announced.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

e-mail: neslihan.toyran@deu.edu.tr Phone: 0232 4124742

Office Hours

Tuesday 12:30-13:30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours) Lectures 12 2 24 Tutorials 12 2 24 Preparations before/after weekly lectures 12 1 12 Preparation for midterm exam 2 3 6 Preparation for final exam 1 3 3 Midterm 2 2 4 Final 1 2 2 TOTAL WORKLOAD (hours) 75

Contribution of Learning Outcomes to Programme Outcomes

PO/LO PO.1 PO.2 PO.3 PO.4 PO.5 PO.6 PO.7 PO.8 PO.9 PO.10 PO.11 PO.12 PO.13 PO.14 PO.15 PO.16 PO.17 PO.18 LO.1 5 5 5 5 5 5 5 5 5 LO.2 5 5 5 5 5 5 LO.3 5 5 5 5 5 5 5 LO.4 5 5 5 5 5 5 5 LO.5 5 5 5 5 5 5 5