COURSE UNIT TITLE

: LABORATORY APPLICATIONS IN MOLECULAR MEDICINE

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
MOT 5011 LABORATORY APPLICATIONS IN MOLECULAR MEDICINE COMPULSORY 1 4 0 8

Offered By

Molecular Medicine

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR DOCTOR GÜL HÜRAY IŞLEKEL

Offered to

Molecular Medicine
Molecular Medicine

Course Objective

The aim of this course is to allow the students to rotate in different laboratories related with the MSc programme in molecular medicine in a multidisciplinary perpective, to learn about the different research methods and techniques used in research laboratory and to acquire practical knowledge in this area. Basic methods learned are as follows: principles of weighing, homogenization techniques, solution preparation, measurement of pH, centrifugation techniques, spectrophotometry, cell culture, gel electrophoresis (agarose, SDS), westernblotting, genomic DNA and total RNA isolation, cDNA synthesis, conventional and real-time PCR, spectrofluorometry, microbiological methods.

Learning Outcomes of the Course Unit

1   To know about the different laboratories which may be involved in molecular medicine
2   To learn about the different subjects and areas of application in these laboratories
3   To understand the basic principles of techniques used in these laboratories
4   To compare different methods in terms of principles and applications
5   To be able to apply these methods in ones own research field
6   To integrate the information acquired and prepare an laboratory notebook

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Course Introduction (T) Laboratory Safety and Principles of the laboratory working, General laboratory equipments, Laboratory Preparation, Laboratory notebook preparation (A)
2 Principles of Weighing , SI Units , Solution Preparation (Concentration Calculations) (T) Measurement of pH, Preparation of buffer solutions (A)
3 Principles of homogenization and centrifugation techniques (T, A)
4 Spectrophotometry (T) Glutathione-S-transferase measurement with spectrophotometer, Protein measurement with Biüret, Lowry, Bradford and BCA methods (A)
5 Spectrofluorometry (T) Measurement of naphtalene with spectrofluorometer(A)
6 Electrophoresis (T) Electrophoresis applications, SDS PAGE, Western Blotting (A)
7 PCR (T) Genomic DNA and Total RNA isolation, cDNA synthesis and conventionall PCR, Real-Time PCR, Agarose gel electrophoresis(A)
8 Cell Culture (T) Cell culture applications (A)
9 Nephelometry, Turbidimetry and Immunochemical Methods (K), (U)
10 Discussion

Recomended or Required Reading

Textbooks:
* Current Protocols in Molecular Biology Copyright © 2007 by John Wiley and Sons, Inc.Last updated: 2 Jul 2009 Editor; Frederick M. Ausubel, Roger Brent, Robert E. Kingston, David D. Moore, J.G. Seidman, John A. Smith, Kevin Struhl
* Nelson DL, Cox MM. Lehninger Principles of Biochemistry. Third editon Worth Publishers, New York, 2000.
* Devlin TM. Texbook of Biochemistry with Clinical Correlations.Fifth edition, Wiley-Liss, New York, 2002.
* Meisenberg G, Simmons WH. Principles of Medical Biochemistry. Mosby Inc., St. Louis, Missouri, 1998.
* Baynes J, Dominiczak MH. Medical Biochemistry. Mosby Inc.,London,1999.
* Marks DB, Marks AD, Smith CM. Basic Medical Biochemistry. A Clinical Approach. Williams & Wilkins, Baltimore, Maryland, 1996.
Yardımcı kaynaklar:
* Onat T, Emerk K, Sözmen E. Insan Biyokimyası, PalmeYayıncılık, Ankara, 2002
* Gürdöl F, Ademoğlu E. Biyokimya, Nobel Tıp Kitabevleri, Istanbul, 2006

Planned Learning Activities and Teaching Methods

The course will be organised as rotations in different labs. There will be short presentations from the instructors on the different methods. Each course will be evaluated by post- test. The students will prepare a laboratory notebook involving the information learned and processed.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 QZ Quiz
3 ASG ASSIGNMENT
4 FCGR FINAL COURSE GRADE (RESIT)
5 FCG FINAL COURSE GRADE VZ * 0.20 + QZ * 0.15 + ODV * 0.15 + FN*0.50
6 RST RESIT
7 BBN BütSonuBaşarıNotu VZ * 0.20 + QZ * 0.15 + ODV* 0.15 + BUT*0.50


Further Notes About Assessment Methods

Each course will be evaluated by post- test. The students will prepare a laboratory notebook involving the information learned and processed.

Assessment Criteria

Understanding, learning, analyse, compare, application, actively contribution

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

e-mail: huray.islekel@deu.edu.tr
Phone: 412 44 08

Office Hours

Tuesday, 12:30-13:30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 14 1 14
Tutorials 14 4 56
Preparations before/after weekly lectures 14 2 28
Preparation for midterm exam 1 10 10
Preparation for final exam 1 30 30
Preparation for quiz etc. 1 10 10
Preparing presentations 2 10 20
Preparing the laboratory notebook 2 16 32
TOTAL WORKLOAD (hours) 200

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11PO.12PO.13PO.14
LO.144553343343335
LO.244555555554555
LO.344554444444555
LO.455555554544555
LO.555555555544555
LO.655555555555445

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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