COURSE UNIT TITLE

: BASIC LABORATORY PRINCIPLES AND METHODS II

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
BIO 6060 BASIC LABORATORY PRINCIPLES AND METHODS II ELECTIVE 2 2 0 9

Offered By

The Institute Of Health Sciences

Level of Course Unit

Third Cycle Programmes (Doctorate Degree)

Course Coordinator

PROFESSOR DOCTOR GÜL HÜRAY IŞLEKEL

Offered to

Audiology
Laboratory Animal Science
Anatomy
Molecular Pathology
Exercise Physiology
Toxicology
Biochemistry
Biomechanics
Histology and Embryology
Medical Biology and Genetics
Biophysics
Physiology
Musculoskeletal Tissue Engineering
Translational Oncology
Medical Parasitology
Microbiology
Pharmacology
Basic Neuroscience
Molecular Medicine
Translational Oncology
Molecular Medicine

Course Objective

The purpose of this course is to teach basic laboratory principles and to introduce advanced laboratory techniques. The course consists of theory and practice sections. In theoretical section of the course, the basic principles and procedures regarding the method are given.
In the implementation stage of the course, in ARLAB, central clinical chemistry lab and research lab of Biochemistry department the students have the chance to introduce all the details of electrophoresis tecniques, atomic absorbtion spectroscopy. They also spend time in liquid chromatography tandem MS lab. For one week, making experiments related to small molecule quantitation. During lab. Sessions the students take notes and prepare lab. Notebook that is evaluated by the coordinator at the end of the course.

Learning Outcomes of the Course Unit

1   Be acquired the knowledge about the basic biochemistry and basic chemistry which are the main areas of biochemistry.
2   Be grasped the theoretical knowledge about the basic biochemistry and basic chemistry which are the main areas of biochemistry.
3   Have the knowledge about the principles and methods of basic laboratory equipments.
4   Be able to critically analyze the basic concepts of advanced laboratory methods (eg, basic differences in analysis of various compound in liquid chromatography tandem Mass Spectrometry)
5   Synthesis: Be able to discuss the learned methods by considering different ways and be able to adapt to new situations (Example: to be able to discuss why different tissues contain different proteins)
6   Be able to present by explicating the results of one of the applied methods.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Course introduction
2 Electrophrosis I
3 Electrophrosis II
4 Electrophrosis III
5 Capillary Electrophrosis
6 GS-MS
7 LC- MS/MS I
8 LC- MS/MS II
9 LC- MS/MS III
10 Isoelectric focusing I
11 Isoelectric focusing II
12 Atomic absorbtion spectroscopy
13 MRM- LC- MS/MS
14 MRM- LC- MS/MS
15 Introduction to proteomics
16 Proteomics in medicine

Recomended or Required Reading

Tietz: Klinik Kimyada Temel Ilkeler Beşinci Baskı. (Editörler: Burtis & Ashwood) (Çeviri Editörü: Diler Aslan).PALMY yayıncılık, pp.352-387.
http://www.ruf.rice.edu/~bioslabs/methods/protein/protein.html
http://www.ruf.rice.edu/~bioslabs/tools/graphs/dataplot.html

Planned Learning Activities and Teaching Methods

This course consists of theory and practical sections. In theoretical section of the course, the basic principles and procedures regarding the method are given. In the implementation stage of the course, in ARLAB, central clinical chemistry lab and research lab of Biochemistry department the students have the chance to introduce all the details of electrophoresis tecniques, atomic absorbtion spectroscopy. They also spend time in liquid chromatography tandem MS lab. For one week, making experiments related to small molecule quantitation.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 ASG ASSIGNMENT
2 FIN FINAL EXAM
3 FCG FINAL COURSE GRADE ASG * 0.30 + FIN* 0.70
4 RST RESIT
5 FCGR FINAL COURSE GRADE (RESIT) ASG * 0.30 + RST* 0.70


Further Notes About Assessment Methods

None

Assessment Criteria

Comprehension, learning, analysis, comparison, applications, interactive participation

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

e-mail : hurayislekel@gmail.com Telefon: 412 44 08

Office Hours

Friday 12.30-13:30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 15 3 45
Tutorials 15 2 30
Preparations before/after weekly lectures 14 2 28
Preparation for final exam 1 40 40
Preparing assignments 2 20 40
Preparing presentations 1 30 30
Preparation for tutorial exam 1 10 10
Final 1 3 3
TOTAL WORKLOAD (hours) 226

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11PO.12PO.13PO.14PO.15PO.16PO.17PO.18PO.19PO.20
LO.133333
LO.233333
LO.333333
LO.433333
LO.533333
LO.633333

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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