COURSE UNIT TITLE

: CELL CULTURE TECHNIQUES

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
TBG 5035 CELL CULTURE TECHNIQUES ELECTIVE 2 2 0 6

Offered By

The Institute Of Health Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR DOCTOR ZEYNEP YÜCE

Offered to

Medical Biology and Genetics
Clinical Neuroscience (Non-Thesis)
Biochemistry
Radiopharmaceutical Sciences
Microbiology
Biomechanics
Molecular Medicine
Histology and Embryology
CLINICAL DRUG RESEARCH
Medical Parasitology
TRANSLATIONAL MEDICINE

Course Objective

Objectives of this course are:
*To acquire basic knowledge on cell culture techniques
*To gain an understanding of the biology and adaptation process of culture cells
*To perform basic cell culture techniques

Learning Outcomes of the Course Unit

1   Outlines the biological features of cells in tissues and cells in culture
2   Recounts the basic knowledge on cell proliferation, transformation, cell death and differentiation
3   Knows the equipment, consumables and culture media used in the cell culture laboratory
4   Knows and practises bio-safety rules, aseptic technique and sterilization procedures in the cell culture laboratory.
5   Describes cloning and transfection techniques
6   Describes cell characterization and separation techniques
7   Prepares culture media; seeds cells in culture; maintains cells in culture; passes/splits suspension and monolayer cultures; freezes and thaws cells; and performs basic viability tests.
8   Monitors culture cells daily and keeps his/her laboratory notebook accordingly.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Introduction The biology of culture cells
2 Design of a cell culture laboratory Instruments, consumables and media used in cell culture techniques
3 Preperation of cell culture media and cell seeding Sterilization procedures, aseptic technique and bio-safety in the cell culture lab
4 Features of primary culture cells and cell lines Splitting suspension culture cells, counting cells and viability tests
5 Contamination in cell culture Freezing protocols Monolayer culture seeding
6 The concepts of transformation and transfection Preparation of freezing media and freezing suspension culture cells
7 Cell cloning, separation and selection methods Splitting monolayer culture cells
8 Characterization of culture cells Differantiaiton of cells in culture Thawing cells
9 Project work on HeLa cell line (discussions and application)
10 Project work on HeLa cell line (discussions and application)
11 Project work on HeLa cell line (discussions and application)
12 Project work on HeLa cell line (discussions and application)
13 Project work on HeLa cell line (discussions and application)
14 Laboratory notebooks and all materials are due

Recomended or Required Reading

Textbook(s):
1- Freshney RI: "Culture of Animal Cells, Amanual of Basic Technique" Fourth ed., Wiley-Liss, 2000
2- Basic Cell Culture Protocols, 2nd ed.: Methods in Molecular Biology, Vol. 75 J.W. Pollard and J.M. Walker, ed., Humana Press, US, 1997
3- Animal Cell Biotechnology: Methods And Protocols N. Jenkins, ed., Humana Press, US, 1999
4- Cell and Tissue Culture for Medical Research A. Doyle and J.B. Griffiths, ed., John Wiley & Sons, Ltd, 2000
Supplementary Book(s):
References:
Materials:

Planned Learning Activities and Teaching Methods

Lectures: Basic theoretical concepts will be explained during lectures
Homework: Students are expected to hand in 4 written reports on topics that are given by the course coordinator.
Project: Each student will monitor, split, maintain, freeze, thaw and perform viability tests on a Hela cells given by the course coordinator during the course. Every observation and application is expected to be recorded in his/her laboratory notebook; which will be handed in at the end of the course.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 PRJ PROJECT
3 FIN FINAL EXAM
4 FCG FINAL COURSE GRADE MTE * 0.40 +PRJ * 0.10 +FIN* 0.50
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.40 +PRJ * 0.10 +RST* 0.50


Further Notes About Assessment Methods

Learning objectives 1-6 will be assessed by a written mid-term exam and by the evaluation of written reports handed in as homework.
Learning objectives 7 and 8 will be assessed by the evaluation of the laboratory notebook handed in by the student; as well as direct observation of his/her lab work. Attendance will also be taken into consideration.
Grading (%):
Mid-term exam: 40%
Homework Assignments/Presentation: 10%
Project & Laboratory work: 50%

Assessment Criteria

To be announced.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

Tel: 412 4608 E-posta: zeynep.sercan@deu.edu.tr

Office Hours

Wednesday s 15:30-17:00

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 14 2 28
Tutorials 14 2 28
Maintaining a cell line 12 1 12
Preparation before/after weekly lectures 14 3 42
Preparation for midterm exam 1 9 9
Preparing Individual Assignments 4 7 28
Midterm 1 3 3
TOTAL WORKLOAD (hours) 150

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8
LO.15
LO.25
LO.354
LO.45
LO.55
LO.645
LO.75
LO.85

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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