COURSE UNIT TITLE

: BASIC LABORATORY PRINCIPLES AND METHODS II

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
BIO 5024 BASIC LABORATORY PRINCIPLES AND METHODS II ELECTIVE 2 2 0 9

Offered By

The Institute Of Health Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR DOCTOR GÜL HÜRAY IŞLEKEL

Offered to

Medical Biology and Genetics
Clinical Neuroscience (Non-Thesis)
The Institute Of Health Sciences
Biophysics
Radiopharmaceutical Sciences
Microbiology
Biomechanics
Molecular Medicine
Basic Nuclear Medicine
Anatomy
Medical Physics
Laboratory Medicine
Medical informatics Scientific Preparatory (Msc)
Medical Parasitology
Biochemistry
Pharmacology
Experimantal Fetal Surgery
Toxicology

Course Objective

The purpose of this course is to teach advanced laboratory principles and related laboratory procedures. The course consists of theory and practice sections. In theoretical section of the course, the basic principles and procedures regarding the method are given.
In the implementation stage of the course, the students both make hands on experiments and observe the running of the biological samples in the laboratory. In the scope of this course LC-MS/MS, GC_MS/MS, IEF, nephelometry, turbidimetry, ELISA etc. Techniques are taught to the students. The students write the procedure and the results on their lab notebook which is evaluated at the end of the semester.

Learning Outcomes of the Course Unit

1   Be acquired the knowledge about the basic biochemistry and basic chemistry which are the main areas of biochemistry.
2   Be grasped the theoretical knowledge about the basic biochemistry and basic chemistry which are the main areas of biochemistry.
3   Have the knowledge about the principles and methods of basic laboratory equipments.
4   Be able to critically analyze the basic concepts of basic laboratory methods
5   Synthesis: Be able to discuss the learned methods by considering different ways and be able to adapt to new situations
6   Be able to present by explicating the results of one of the applied methods.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Introduction, Course Introduction
2 Liquid Chromatography (HPLC)
3 Liquid Chromatography (HPLC)
4 RIA, ELISA
5 GC-MS
6 Basic principles of Electrophoresis
7 Capillary Electrophoresis
8 IEF
9 IEF
10 Basic Principles and Applications of Atomic Absorbtion Spectrophotometer;
11 Basic Principles of Liquid Chromatography Tandem Mass Spectometry, application
12 Quantitation in MRM mode with Liquid Chromatography Tandem Mass Spectometry
13 Introduction to proteomics
14 Applications of proteomics in medicine

Recomended or Required Reading

Textbook(s):
1. DNA Repair and mutagenesis, Errol C. Friedberg, Graham C. Walker, Wolfram Siede,Richard D. Wood, Roger A.Schultz and Tom Ellenberger Second Edition ASM Press, Washington DC, 2006
2. Mass Spectrometry, Edmond deHoffmann and Vincent Stroobant Second Edition John Wiley, Chichester, 2002,
3. Tietz: Klinik Kimyada Temel Ilkeler Beşinci Baskı. (Editörler: Burtis & Ashwood) (Çeviri Editörü: Diler Aslan).PALMY yayıncılık, pp.352-387.

Planned Learning Activities and Teaching Methods

This course consists of theory and practical sections. In theoretical section of the course, the basic principles and procedures regarding the methoda are given. In practical section the students the students both make hands on experiments and observe the running of the biological samples in the laboratory. The students write the procedure and the results on their lab notebook which is evaluated at the end of the semester.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 ASG ASSIGNMENT
2 FIN FINAL EXAM
3 FCG FINAL COURSE GRADE ASG * 0.30 + FIN* 0.70
4 RST RESIT
5 FCGR FINAL COURSE GRADE (RESIT) ASG * 0.30 + RST* 0.70


Further Notes About Assessment Methods

None

Assessment Criteria

Comprehension, learning, analysis, comparison, applications, interactive participation

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

e-mail : huray.islekel@deu.edu.tr Telefon: 412 44 08

Office Hours

Friday 12.30-13:30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 14 3 42
Tutorials 14 2 28
Preparations before/after weekly lectures 14 3 42
Preparation for final exam 1 35 35
Preparing presentations 1 25 25
Preparing assignments 2 25 50
Final 1 3 3
TOTAL WORKLOAD (hours) 225

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9
LO.15
LO.24
LO.34
LO.45
LO.55
LO.65

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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