COURSE UNIT TITLE

: CELL CULTURE TECHNOLOGY

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
BYM 0503 CELL CULTURE TECHNOLOGY ELECTIVE 3 0 0 8

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Third Cycle Programmes (Doctorate Degree)

Course Coordinator

PROFESSOR DOCTOR ISMET GÜRHAN DELILOĞLU

Offered to

Ph.D. in Biotechnology
Industrial Ph.D. Program In Advanced Biomedical Technologies
Industrial Ph.D. Program In Advanced Biomedical Technologies
Ph.D. in Biotechnology
BIOTECHNOLOGY

Course Objective

This course is mainly for the students who have no background about the subject during their undergraduate education. The aim of the course is to create an approach on the animal (human) cell/tissue culture technology in general.

Learning Outcomes of the Course Unit

1   Fundamental conception information of animal cell and tissue culture techniques.
2   Comprehend the importance and application range of animal cell and tissue culture techniques.
3   Making the students conscious of animal cell culture technology to be able to carry out researches

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Basic technical words/expressions, Historical development of tissue culture technology Lecturing
2 Setting up a cell culture laboratory, operating principals, Lecturing
3 Laboratory safety conciderations and Visualisation of animal cell cultures Lecturing
4 Functions, preparations and sterilisation of the growth media Lecturing
5 Primary cell culture preparation Lecturing
6 Diploid and heteroploid cell cultures Lecturing
7 Midterm exam Lecturing
8 Cryopreservation and transpotation of the cell cultures, cell banks & culture collections Lecturing
9 Characterisation and Quantitative procedures, cell viability determination Lecturing
10 Cell transfection procedures Lecturing
11 3D cultures Lecturing
12 Problems and troubleshooting Lecturing
13 Artical evaluation Lecturing
14 Artical evaluation Lecturing

Recomended or Required Reading

Culture of Animal Cells: A manual of Basic Techniques, by Freshney, I.A., 2005, Wiley-Liss, Inc., New York. Handbook of Industrial Cell Culture: Mammalian, Microbial, and
Plant Cells (Hardcover) by Victor A. Vinci (Editor), Sarad R. Parekh (Editor), 2003, Humana Press Inc. Cell of Cells: The Global Race to Capture and Control the Stem Cell
(Hardcover) by Cynthia Fox (Author), 2007, W.W. Worton&Company Inc. Embryonic Stem Cells: A Practical Approach (Practical Approach Series) (Paperback)by Elena Notarianni
(Editor), Martin J. Evans (Editor), 2006, Oxford University Press Mesenchymal Stem Cells: Methods and Protocols (Methods in Molecular Biology) (Hardcover) by Darwin J.
Prockop (Editor), Donald G. Phinney (Editor), Bruce A. Bunnell (Editor),2008, Humana Press. Animal Cell Culture and Technology, Buttler, M., (2004) BIOS Scientific.

Planned Learning Activities and Teaching Methods

homework and project

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 FIN FINAL EXAM
4 FCG FINAL COURSE GRADE MTE * 0.20 + ASG * 0.20 + FIN * 0.60
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.20 + ASG * 0.20 + RST * 0.60


Further Notes About Assessment Methods

None

Assessment Criteria

To be announced.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

Prof. Dr. S. Ismet Deliloğlu Gürhan

Office Hours

To be announced.

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 12 3 36
Preparation for midterm exam 1 30 30
Preparation for final exam 1 40 40
Preparing assignments 5 15 75
Reading 12 2 24
Midterm 1 2 2
Final 1 2 2
TOTAL WORKLOAD (hours) 209

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6
LO.1555455
LO.2555455
LO.3555555