Dokuz Eylül University Information Package / Courses Catalog

Description of Individual Course Units

Course Unit Code	Course Unit Title	Type Of Course	D	U	L	ECTS
BYT 5005	HPLC IN ANALYSIS OF BIOLOGICAL MOLECULES	ELECTIVE	3	0	0	8

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR DOCTOR HÜLYA AYAR KAYALI

Offered to

Ph.D. in Biotechnology
Ph.D. in Biotechnology
BIOTECHNOLOGY

Course Objective

HPLC is a chromatographic technique used to separate a mixture of compounds with the purpose of identifying,quantifying and purifying the individual components of the mixture.HPLC method has been used for qualitative and quantitative determination of drug,pesticide residues,hormone and vitamine levels as well as other metabolites in biological samples.The course aims to provide an introduction and applicabilty of HPLC,which is a technique for the separation and determination of organic and inorganic solutes in any samples especially biological,pharmaceutical,food,environmental,industrial.

Learning Outcomes of the Course Unit

1	Comprehend the basic principle of HPLC,
2	Realize the column, detecter used in HPLC and differences of these,
3	Study sample preparation techniques for HPLC,
4	Be carried out biochemical applications and develop a method in HPLC,
5	Realize other technique combinations as MS with HPLC.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week	Subject	Description
1	Introduction to the HPLC
2	Principles and Terms of HPLC
3	Basics of Separations
4	Detection Sensitivity and Selectivity
5	Sample Preparation and Column
6	Non-ionic samples: Reversed and Normal Phase HPLC
7	Ionic samples: Reversed Phase, ion pair, ion exchanger HPLC
8	Midterm Exam
9	Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates and Related Compounds
10	Special Needs of Biochemical HPLC
11	Peptide and Protein Separation
12	Ion Exchanger HPLC
13	Oligonucleotide Separation
14	Size-Exclusion Chromatography

Recomended or Required Reading

Textbook(s): Snyder, L. R., Kirkland, J. J. & Glach, J. L. Practical HPLC Method Development A Wiley-Interscience Publication, 1997 .
Supplementary Book(s): Snyder, L. R., Kirkland, J.J. & Dolan, J. W. Introduction To Modern Liquid Chromatography
References:
Materials:

Planned Learning Activities and Teaching Methods

1. Presentation
2. Interactive narrative
3. Question-answer technique
4. Homework

Assessment Methods

SORTING NUMBER	SHORT CODE	LONG CODE	FORMULA
1	MTE	MIDTERM EXAM
2	ASG	ASSIGNMENT
3	PAR	PARTICIPATION
4	FIN	FINAL EXAM
5	FCG	FINAL COURSE GRADE	MTE* 0.30 + ASG * 0.20 + PAR * 0.20 + FIN * 0.30
6	RST	RESIT
7	FCGR	FINAL COURSE GRADE (RESIT)	MTE* 0.30 + ASG * 0.20 + PAR * 0.20 + RST * 0.30

Further Notes About Assessment Methods

None

Assessment Criteria

Meaningful learning of the basic concepts given in presentations, association of concepts with each other, establishing the cause-result relationships and making comments by using the information available for problems and evaluating idea generation are carried out with mid-term and final exams, active participation to the lecture, homework.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

Dokuz Eylül University, Science Faculty, Chemistry Department, Biochemistry Division, 35160, Buca, Izmir, Türkiye
e-mail:hulya.kayali@deu.edu.tr
Tel: +90 232 3018690

Office Hours

Wednesday 13.30-15.30

Work Placement(s)

None

Workload Calculation

Activities	Number	Time (hours)	Total Work Load (hours)
Lectures	13	3	39
Preparation before/after weekly lectures	13	3	39
Preparation for Mid-term Exam	1	20	20
Preparation for Final Exam	1	25	25
Preparing Individual Assignments	3	10	30
Preparing Presentations	3	12	36
Final	1	2	2
Midterm	1	2	2
TOTAL WORKLOAD (hours)			193

Contribution of Learning Outcomes to Programme Outcomes

PO/LO	PO.1	PO.2	PO.3	PO.4	PO.5	PO.6
LO.1	5	3	5	3	4	5
LO.2	5	3	5	3	4	5
LO.3	5	3	5	3	4	5
LO.4	5	4	5	4	5	5
LO.5	5	4	5	4	5	5

COURSE UNIT TITLE

DEGREE PROGRAMMES

Description of Individual Course Units

Offered By

Level of Course Unit

Course Coordinator

Offered to

Course Objective

Learning Outcomes of the Course Unit

Mode of Delivery

Prerequisites and Co-requisites

Recomended Optional Programme Components

Course Contents

Recomended or Required Reading

Planned Learning Activities and Teaching Methods

Assessment Methods

Further Notes About Assessment Methods

Assessment Criteria

Language of Instruction

Course Policies and Rules

Contact Details for the Lecturer(s)

Office Hours

Work Placement(s)

Workload Calculation

Contribution of Learning Outcomes to Programme Outcomes

CONTACT INFORMATION