COURSE UNIT TITLE

: FLUORESCENCE SPECTROSCOPY IN ANALYTICAL CHEMISTRY

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
KIM 5054 FLUORESCENCE SPECTROSCOPY IN ANALYTICAL CHEMISTRY ELECTIVE 3 0 0 8

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR DOCTOR ÖZLEM ÖTER

Offered to

Chemistry
Chemistry
Chemistry

Course Objective

Fluorescence techniques are at the heart of uncountable applications found in simple, commonplace instruments to throughly investigative, advanced tools in modern scientific research. Fluorescence spectroscopy in Analytical Chemistry will not only cover the basics of fluorescence techniques, but also guide the participants towards the successfull utilization of the newest methodologies, instruments, fluorescent dyes and sensor technologies.

Learning Outcomes of the Course Unit

1   To have the ability to follow latest developments in the field, to improve current information and to propose new ideas and methods for researches.
2   To have the ability to use modern technologies for solving problems in chemistry.
3   To have the ability to contribute to the new structures supported by Research & Development in industry- university collaborations.
4   To have the consciousness of professional responsibility and to have problem-solving ability.
5   To have the ability to work in production related areas of chemistry.
6   To have the ability to generate solutions and to think critically and analytically.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Basic Definitions and Principles of Fluorescence Basic Spectral Properties Jablonski Diagram and Stokes' shift Excitation and Emission Spectra Fluorescence Lifetime FRET: Fluorescence Resonance Energy Transfer
2 Instrumentation Steady-State Fluorometer Instrumentation for Time-Resolved Fluorescence Light Sources for Time-Resolved Fluorometry (lamps, lasers, laser diodes, LEDs, synchrotron radiation) Detectors (PMT, APD, MCP)
3 Fluorophores Chemical Sensing probes Some special probes Quantum dots
4 Solvent Effects on Emission Spectra
5 Quenching of Fluorescence Quenchers of fluorescence Theory of collisional quenching Theory of static quenching Intramolecular quenching
6 Time-dependent Phenomena (Part I) Multi-Exponential Decays Time-Domain Lifetime Measurements Frequency-Domain Lifetime Measurements Quenching, Static, Dynamic, Transients
7 Analytical Applications of Fluorescence Advantages of Fluorescence in Chemical Analysis Examples of Fluorescence Assays Methods of Fluorescence Sensing
8 Fluorescence Lifetime-Based Sensing Lifetime Sensing and Ratiometric Probes and Midterm Exam Fiber Optics Sensors
9 Student Presentations and evaluation of given projects
10 Student Presentations and evaluation of given projects
11 Student Presentations and evaluation of given projects
12 Student Presentations and evaluation of given projects
13 Student Presentations and evaluation of given projects
14 Student Presentations and evaluation of given projects

Recomended or Required Reading

TEXT BOOKS:
Principles of Fluorescence Spectroscopy, Joseph R. Lakowicz, Kluwer Academic, New York, 1999.

REFERENCE BOOKS:
Structure and Dynamics of Macromolecules: Absorption and Fluorescence Studies, J.R. Albani, Elsevier, 2004, Pages 141-192.

Principles and Applications of Fluorescence Spectroscopy , Albani, Jihad Rene, John Wiley & Sons, Ltd, 2008

Topics in Fluorescence Spectroscopy, Volume 4 : Probe Design and Chemical Sensing , Lakowicz, J. R., Kluwer Academic Publishers, 1994.

Planned Learning Activities and Teaching Methods

The course is taught in a lecture, class presentation and discussion format. All class members are expected to attend both the lecture and seminar hours and take part in the discussion sessions. Besides the taught lecture, group presentations are to be prepared by the groups assigned for that week and presented to open a discussion session.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 FCG FINAL COURSE GRADE MTE * 0.50 + ASG * 0.50


Further Notes About Assessment Methods

None

Assessment Criteria

To be announced.

Language of Instruction

English

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

Assoc. Prof. Dr. Ozlem Oter
e-mail: ozlem.oter@deu.edu.tr, ozlem.oter@hotmail.com
Tel: 0232 301 95 06

Office Hours

To be announced.

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 8 3 24
Presentations 6 3 18
Tutorials 14 5 70
Preparation before/after weekly lectures 14 2 28
Preparing presentations 2 10 20
Preparing group assignments 2 10 20
Preparing for midterm/final exam 1 18 18
Midterm/Final 1 2 2
TOTAL WORKLOAD (hours) 200

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11
LO.1334333
LO.23544
LO.335
LO.45
LO.55
LO.65

CONTACT INFORMATION
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Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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