COURSE UNIT TITLE

: TLC APPLICATIONS IN ANALYTICAL CHEMISTRY

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
KIM 5038 TLC APPLICATIONS IN ANALYTICAL CHEMISTRY ELECTIVE 3 0 0 7

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR DOCTOR MELEK MERDIVAN

Offered to

Chemistry
Chemistry
Chemistry

Course Objective

The course aims to explain the mechanism of thin layer chromatography (TLC) and introduce the extensive use of TLC in chemical, environmental, forensic and pharmaceutical analysis and provide to instruct the use TLC-densitometry equipment.

Learning Outcomes of the Course Unit

1   To be able to explain the mechanism and theory of TLC, chromatographic performance, high performance TLC.
2   To be able to understand all steps in the analytical procedure, identify the compounds analyzed.
3   To be able to evaluate data obtained with TLC technique and interpret the results obtained.
4   To be able to choose the correct materials for TLC and learn how TLC-densitometry will be used.
5   To be able to learn the usefullness of TLC as qualitative and quantitative in the large application areas such as pharmaceutical, food and environmental analysis.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Classification of TLC, criteria of chromatographic performance, High performance TLC
2 Stationary phases for TLC; adsorption, partitio, ion exchange, size exclusion
3 Sample preparation; obtaining material, storage, preservation and freezing
4 Preparation of samples for application, preparation of plate prior to spotting, sample application for TLC, sample application for HPTLC
5 Choice of mobile phase, troubleshooting separation problems, Selection of mobile phase for HPLC
6 Chambers and instrument, effect of temperature, gradient elution
7 Ultraviolet lamps, techniques for visualization with detection reagents, chromogenic and fluoregenic detection reagents, derivative formation
8 Identification of compounds, storage of chromatograms, scraping and elution, visual comparison, area measurement, in situ densitometry
9 Reproducibility of results; nature of sorbent, contamination of sorbents and solvents, water content, sample application and sample size, chromatographic development
10 TLC of organic dyes
11 TLC of pigments and vitamins
12 Pharmaceuticals and metal analysis
13 Forensic applications
14 Presentation of homework

Recomended or Required Reading

Fried, B., Sherma, J. (eds) : Thin-Layer Chromatography, Marcel Dekker Inc., New York, 1996.
Fried, B., Sherma, J. (eds) : Practical TLC: A Multidisciplinary Approach, CRC Pres Inc., 1996.
Ahuja, S. (ed): Chromatography and Separation Science, Academic press, California, 2003.

Planned Learning Activities and Teaching Methods

Lecture, class presentation, homework, question-answer technique

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 ASG ASSIGNMENT
2 FIN FINAL EXAM
3 FCG FINAL COURSE GRADE ASG * 0.50 + FIN * 0.50
4 RST RESIT
5 FCGR FINAL COURSE GRADE (RESIT) ASG * 0.50 + RST * 0.50


Further Notes About Assessment Methods

None

Assessment Criteria

Assessment of the students will be done by using homework and final examination.

Language of Instruction

English

Course Policies and Rules

Attendance to at least 70% for the lectures is an essential requirement of this course and is the responsibility of the student. It is necessary that attendance to the lecture and homework delivery must be on time. Any unethical behavior that occurs either in presentations or in exams will be dealt with as outlined in school policy. You can find the undergraduate policy at http://web.deu.edu.tr/fen

Contact Details for the Lecturer(s)

DEU Science Faculty Chemistry Deparment
e-mail: melek.merdivan@deu.edu.tr
Tel: 0 232 3018693

Office Hours

To be announced.

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 14 3 42
Preparation before/after weekly lectures 14 5 70
Preparation for Final Exam 1 35 35
Preparation for homework 1 30 30
Presentation of homework 1 3 3
Final exam 1 3 3
TOTAL WORKLOAD (hours) 183

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11
LO.133
LO.2
LO.33
LO.43
LO.53

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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