COURSE UNIT TITLE

: MOLECULAR MICROBIOLOGY TECHNIQUES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
BYL 6024 MOLECULAR MICROBIOLOGY TECHNIQUES ELECTIVE 3 0 0 6

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Third Cycle Programmes (Doctorate Degree)

Course Coordinator

PROFESSOR DOCTOR ARZU GÖRMEZ

Offered to

Ph.D. in Biology
Biology

Course Objective

The aim of this course is to teach the current techniques used in molecular biology.

Learning Outcomes of the Course Unit

1   learns biological molecule extraction, detection, and analysis methods.
2   can give information about current molecular methods.
3   can comprehend the strengths and weaknesses of different experimental approaches used in molecular microbiology.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 An Overview of Methods Used in Molecular Biology
2 Model Organisms/ Cultured cells and viruses
3 Amplification of DNA and RNA sequences/DNA cloning
4 Undirected genome manipulation
5 Directed genome manipulation
6 Detection of biological molecules
7 Seperation and isolation of biological molecules
8 Identifying the composition of biological molecules
9 Midterm exam
10 Obtaining and analyzing sequences on a genomic scale
11 Detection of specific DNA and RNA sequences
12 Detection of specific proteins
13 Detection of interactions between molecules
14 Genome-wide detection of interaction between molecules / Molecular structure determination

Recomended or Required Reading

1. Nancy L Craig, Orna Cohen-Fix, Rachel Green, Carol W Greider, Gisela Storz, Cynthia Wolberge, Molecular Biology Principles of Genome Function, Published in the United States by Oxford University Press Inc., New York.
2. Brown, T.A., 2016. Gene Cloning and DNA Analysis: An Introduction, 7th Edition. John Wiley ve Sons, Ltd. Published 2016 by John Wiley ve Sons, Ltd.
3. Temizkan, G., Arda, N., 2021 Basic and Advanced Molecular Biology Methods Genomic and Proteomic Analysis, 2nd Edition Nobel Medicine, Istanbul.

Planned Learning Activities and Teaching Methods

The course is taught in a lecture, class presentation, and discussion format. All class members are expected to attend the lecture and participate in the discussion sessions. Besides the course, group presentations are to be prepared by the groups assigned for that week and presented to open a discussion session.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 ASG ASSIGNMENT
2 MTE MIDTERM EXAM
3 FIN FINAL EXAM
4 FCG FINAL COURSE GRADE ASG * 0.30 + MTE * 0.30 + FIN * 0.40
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) ASG * 0.30 + MTE * 0.30 + RST * 0.40


Further Notes About Assessment Methods

None

Assessment Criteria

The student is evaluated with a midterm exam, research presentations, and a final assignment.

Language of Instruction

Turkish

Course Policies and Rules

It is the student's responsibility to attend 70% of the courses during the semester. Students are required to be present during the lesson and on the days when the presentation is required. Regarding unethical behaviors that may occur in classes and exams, action is taken within the framework of the relevant regulation. The regulation on the teaching and examination application principles of the DEU Institute of Science and Technology can be obtained from the address http://fbe.deu.edu.tr/.

Contact Details for the Lecturer(s)

arzu.gormez@deu.edu.tr

Office Hours

It will be announced.

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 13 3 39
Student Presentations 3 1 3
Preparations before/after weekly lectures 13 2 26
Preparation for midterm exam 1 15 15
Preparing presentations 3 4 12
Preparing assignments 1 20 20
Web Search and Library Research 3 8 24
Project Final Presentation 1 2 2
Midterm 1 1 1
Project Assignment 1 2 2
TOTAL WORKLOAD (hours) 144

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11PO.12
LO.1545354355533
LO.254535535554
LO.355545543553