COURSE UNIT TITLE

: THE MOLECULAR TECHNIQUES AND SCIENTIFIC RESEARCH USED IN IDENTIFICATION OF MICROORGANISMS

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
ELECTIVE

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR DOCTOR ARZU GÖRMEZ

Offered to

Ph.D. in Biology
Biology

Course Objective

This course aims to inform students about microorganisms and their identification by molecular methods.

Learning Outcomes of the Course Unit

1   to explain the cell structures and mechanisms of microorganisms.
2   to have knowledge about the identification of microorganisms by classical and molecular methods.
3   to learn current scientific methods related to molecular methods used in identifying microorganisms.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 General information about microorganisms I- Cell structure of procaryote and eucaryote.
2 General information about microorganisms II: Groups of microorganisms
3 Classical methods for identification of microorganisms (morphological, physiological, biochemical tests, etc.)
4 Serological and molecular methods for the identification of microorganisms
5 Fatty Acid Methyl Ester (FAME) profiling (Microbial Identification System), Metabolic (carbon profiling) fingerprint (BIOLOG)
6 Genetic profiling, MLST analysis
7 Electrophoretic and spectrophotomethric techniques
8 Chromotographic techniques
9 Midterm Exam
10 Recombinant DNA techniques - Gene cloning
11 rDNA sequences, Phylogenetic tree, SPSS analysis
12 Hybridization methods
13 Proteomic studies for microbiology, Microarray
14 Scientific Research and Experimental Applications

Recomended or Required Reading

1. Molecular Microbiology: Diagnostic Principles and Practices / David H. Persing, Fred C. Tenover, Randall T. Hayden, Margareta Ieven, Melissa B. Miller, Frederick S. Nolte, Yi-Wei Tang, Alex van Belkum, ASM Press.
2. Brock Biology of Microorganisms 16th Ed., Madigan M.T., Kelly S. Bender, W Matthew Sattley, David A. Stahl. Dunlap P.V., Clark D.P. Pearson Education Inc.
3. Molecular Genetics of Bacteria. 5th Edition. Jeremy W. Dale, Simon F. Park, Wiley Blackwell.

Planned Learning Activities and Teaching Methods

The course is taught in a lecture, class presentation, and discussion format. All class members are expected to attend the lecture and participate in the discussion sessions. Besides the course, group presentations are to be prepared by the groups assigned for that week and presented to open a discussion session.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 ASG ASSIGNMENT
2 MTE MIDTERM EXAM
3 FIN FINAL EXAM
4 FCG FINAL COURSE GRADE ASG * 0.30 + MTE * 0.30 + FIN * 0.40
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) ASG * 0.30 + MTE * 0.30 + RST * 0.40


Further Notes About Assessment Methods

None

Assessment Criteria

The student is evaluated with a midterm exam, research presentations, and a final project assignment.

Language of Instruction

Turkish

Course Policies and Rules

It is the student's responsibility to attend 70% of the courses during the semester. Students are required to be present during the lesson and on the days when the presentation is required. Regarding unethical behaviors that may occur in classes and exams, action is taken within the framework of the relevant regulation. The regulation on the teaching and examination application principles of the DEU Institute of Science and Technology can be obtained from the address http://fbe.deu.edu.tr/.

Contact Details for the Lecturer(s)

arzu.gormez@deu.edu.tr

Office Hours

It will be announced.

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 13 3 39
Student Presentations 3 1 3
Preparations before/after weekly lectures 13 2 26
Preparation for midterm exam 1 15 15
Preparing assignments 1 20 20
Web Search and Library Research 3 8 24
Preparing presentations 3 4 12
Final Assignment 1 2 2
Midterm 1 1 1
Project Final Presentation 1 2 2
TOTAL WORKLOAD (hours) 144

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11PO.12
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LO.254555455455
LO.355555455555

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

Copyright 2015 DEÜ. BİD

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