COURSE UNIT TITLE

: BASIC CELL AND TISSUE CULTURE TECHNIQUES

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
ELECTIVE

Offered By

Chemistry

Level of Course Unit

First Cycle Programmes (Bachelor's Degree)

Course Coordinator

PROFESSOR DOCTOR HÜLYA AYAR KAYALI

Offered to

Chemistry

Course Objective

Within the scope of this course; It is aimed that students learn the basic principles of cell and tissue culture, design of tissue culture laboratory, special devices and materials used, safety in tissue culture laboratories, working principles in tissue culture laboratories.

Learning Outcomes of the Course Unit

1   Comprehend the importance, basic terminology, and principles of cell and tissue culture.
2   Gain knowledge about the laboratory infrastructure, equipment, and materials where cell culture studies will be carried out and can generate ideas.
3   Be knowledgeable about the application of basic techniques used in cell culture, media, sterilization techniques, and contamination risks.
4   Evaluate images and gain knowledge of microscopic imaging techniques.
5   Gain knowledge of primary cultures and cell lines creation, cloning, and scaling in cell culture.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Introduction to the history and basic terminology of cell and tissue culture.
2 Cell culture laboratory setup; space requirement, equipment, plastic, and other disposable materials, maintenance of the laboratory.
3 physical environment; temperature, pH, osmolarity, CO2 and other gases Surface and cell shapes; adherent and non-adherent cells.
4 Sterilization; wet heating, dry heating, irradiation, chemical sterilization, filtration.
5 Media in cell culture; basic components in cell culture media, preparation of culture media.
6 Standard cell culture techniques; subculturing of adhered and suspended cultures, growth curves and measurement of cell growth, freeze-thaw.
7 Microscopy of living cells; general imaging methodology, imaging of intact cell cultures in flasks, Petri dishes, and plates, high-resolution imaging of cells on coverslips, imaging of fluorescently labeled cells, recording and analysis of microscopic images.
8 Midterm
9 contamination; prevention and detection, detection of mycoplasmas by fluorescent method, cross-culture contamination.
10 Development and optimization of serum and protein-free culture media.
11 Establishment of primary cultures and cell lines.
12 Specific cell types and requirements
13 Cell cloning procedures; technique selection, techniques for adherent and suspended cells.
14 Cell culture for commercial applications; industrial-grade cells; small and large scales.

Recomended or Required Reading

Main Source: John M. Davis, Basic Cell Culture: A Practical Approach, Oxford University Press, (2002).
Helpful resources: John M. Davis, Animal Cell Culture: Essential Methods, Wiley-Blackwell, (2011).
P. R. Yadav, Cell Culture, Discovery Publishing House, (2005).
R. Ian Freshney, Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, John Wiley & Sons, (2011).
Jennie P. Mather, Penelope E. Roberts, Introduction to Cell and Tissue Culture: theory and technique, Springer, (1998).

Planned Learning Activities and Teaching Methods

Presentation
Question and answer technique
Practice
Homework

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 PAR PARTICIPATION
4 FIN FINAL EXAM
5 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.30 + ASG * 0.20 + PAR * 0.10 + FIN * 0.40
6 RST RESIT
7 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.30 + ASG * 0.20 + PAR * 0.10 + RST * 0.40


Further Notes About Assessment Methods

None

Assessment Criteria

Meaningful learning of the basic concepts given in the presentations, associating the concepts with each other, establishing cause-effect relationships, making comments on questions or problems using their existing knowledge, and evaluating the production of ideas will be carried out with midterm, final exam, active participation in the lesson, and assignments.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

DEU Faculty of Science, Department of Chemistry
e-posta: hulya.kayali@deu.edu.tr

Office Hours

Wednesday 15.00-17.00

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 13 2 26
Preparations before/after weekly lectures 5 1 5
Preparation for midterm exam 1 10 10
Preparation for final exam 1 15 15
Preparing presentations 1 15 15
Final 1 2 2
Midterm 1 2 2
TOTAL WORKLOAD (hours) 75

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11PO.12PO.13PO.14PO.15PO.16PO.17
LO.133343
LO.223343
LO.33333
LO.4323333
LO.533333

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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