COURSE UNIT TITLE

: BASIC LABORATORY PRINCIPLES AND METHODS I

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
BIO 6059 BASIC LABORATORY PRINCIPLES AND METHODS I ELECTIVE 1 4 0 9

Offered By

The Institute Of Health Sciences

Level of Course Unit

Third Cycle Programmes (Doctorate Degree)

Course Coordinator

PROFESSOR DOCTOR GÜLGÜN OKTAY

Offered to

Audiology
Laboratory Animal Science
Anatomy
Molecular Pathology
Exercise Physiology
Toxicology
Biochemistry
Biomechanics
Doctorate Program Rare Diseases
Histology and Embryology
Medical Biology and Genetics
Biophysics
Physiology
Musculoskeletal Tissue Engineering
Translational Oncology
Medical Parasitology
Microbiology
Pharmacology
Basic Neuroscience
Molecular Medicine
Translational Oncology
Molecular Medicine

Course Objective

The purpose of this course is to teach basic laboratory principles and general laboratory procedures and also to introduce separation techniques. The course consists of theory and practice sections. This course is given as a mini project. In theoretical section of the course, the basic principles and procedures regarding the method are given.
In the implementation stage of the course, the supernatants are obtained from rat tissues (brain, liver, heart, kidney, lung) by weighing, homogenization and centrifugation. The supernatants obtained are used in all methods to learn throughout the semester. In these supernatants primarily the protein concentrations are determined using spesific protein methods like Biuret, Modified Lowry and Bradford.
Then, Glutathione-S-transferase and MDA levels are measured by using a spectrophotometer, spectrofluorimeter and Liquid Chromatography (HPLC) method, respectively. The molecular weights "MW" of unknown protein bands are calculated by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis).The is Students make presentations related with their results in the end of the semester.

Learning Outcomes of the Course Unit

1   Be acquired the knowledge about the basic biochemistry and basic chemistry which are the main areas of biochemistry.
2   Be grasped the theoretical knowledge about the basic biochemistry and basic chemistry which are the main areas of biochemistry.
3   Have the knowledge about the principles and methods of basic laboratory equipments.
4   Be able to critically analyze the basic concepts of basic laboratory methods (eg, such as differences in methods of protein determination)
5   Synthesis: Be able to discuss the learned methods by considering different ways and be able to adapt to new situations (Example: to be able to discuss why different tissues contain different proteins)
6   Be able to present by explicating the results of one of the applied methods.
7   Be acquired the basic laboratory skills ( for example: to weigh, buffer preparation, pH measurement, to make homogenize, to make centrifuge)
8   Be acquired the application skills of the general laboratory prosedures

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 * Introduction * Course Introduction * Laboratory Safety * Principles of the laboratory working * General Laboratory Equipment
2 * Laboratory Preparation * Principles of Weighing * SI Units * Solution Preparation (Concentration Calculations)
3 * Solution Preparation (Concentration Calculations) * Measurement of pH and buffer preparation
4 * Project Preparatory Work * Homogenization techniques * Centrifugation, the basic principles and types * Video display
5 * Spectrophotometry; Basic Principles and Applications * Glutathione-S-transferase measurement with spectrophotometer
6 Protein content measurement using a spectrophotometer * Protein determination by Biüret method * Protein determination by Lowry Method
7 * Protein Determination content by Bradford Method * Protein determination by the BCA Method
8 Group presentations ; Protein determination methods practice
9 MIDTERM EXAM Written and Practice Exam
10 * Spectrofluorimeter; Basic Principles and Applications * Spectrofluorimeter with the measurement of MDA
11 * Basic Principles of Chromatography * Types and Applications of Chromatography
12 * Liquid Chromatography (HPLC) method with the MDA measurement
13 Basic Principles of electrophoresis * Types and Applications of Electrophoresis * Preparation of Electrophoresis Buffer * Quantification of protein bands * Gel cast * Gel Execution * Gel Paint
14 * Basic principles of Zymography methods *Types of Zymography methods *Application of Gelatin zymography method * Application of Casein zymography method * Application of Collagen zymography method

Recomended or Required Reading

Textbook(s):
Tietz: Klinik Kimyada Temel Ilkeler Beşinci Baskı. (Editörler: Burtis & Ashwood) (Çeviri Editörü: Diler Aslan).PALMY yayıncılık, pp.352-387.
http://www.ruf.rice.edu/~bioslabs/methods/protein/protein.html
http://www.ruf.rice.edu/~bioslabs/tools/graphs/dataplot.html

Planned Learning Activities and Teaching Methods

This course consists of theory and practical sections. This course is given as a mini project. In theoretical section of the course, the basic principles and procedures regarding the method are given.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 FIN FINAL EXAM
3 FCG FINAL COURSE GRADE MTE * 0.50 + FIN* 0.50
4 RST RESIT
5 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.50 + RST* 0.50


Further Notes About Assessment Methods

None

Assessment Criteria

Comprehension, learning, analysis, comparison, applications, interactive participation

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

gulgun.oktay@deu.edu.tr Telefon: 412 44 02 GSM: + 5363548774

Office Hours

Friday 12.30-13:30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 15 3 45
Tutorials 30 2 60
Preparations before/after weekly lectures 14 2 28
Preparation for midterm exam 1 20 20
Preparation for final exam 1 40 40
Preparing assignments 1 18 18
Preparing presentations 1 10 10
Final 1 3 3
Midterm 1 3 3
TOTAL WORKLOAD (hours) 227

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11PO.12PO.13PO.14PO.15PO.16PO.17
LO.13
LO.23
LO.35
LO.43
LO.53
LO.64
LO.73
LO.84

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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