COURSE UNIT TITLE

: CELL CULTURE TECHNIQUES

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
TBG 6095 CELL CULTURE TECHNIQUES ELECTIVE 2 4 0 12

Offered By

The Institute Of Health Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR DOCTOR ZEYNEP YÜCE

Offered to

Audiology
Laboratory Animal Science
Anatomy
Molecular Pathology
Exercise Physiology
Toxicology
Biochemistry
Biomechanics
Histology and Embryology
Medical Biology and Genetics
Biophysics
Physiology
Musculoskeletal Tissue Engineering
Translational Oncology
Medical Parasitology
Microbiology
Pharmacology
Basic Neuroscience
Molecular Medicine
Translational Oncology
Molecular Medicine

Course Objective

Objectives of this course are:
*To acquire advance knowledge on cell culture techniques
*To conceptually understand of the biology and adaptation process of culture cells
*To perform intermediate/ advance cell culture techniques

Learning Outcomes of the Course Unit

1   Discusses the biological features of cells in tissues and cells in culture
2   Explains the mechanisms of cell proliferation, transformation, cell death and differentiation
3   Knows in detail the equipment, consumables and culture media used in the cell culture laboratory
4   Knows practises and teaches bio-safety rules, aseptic technique and sterilization procedures in the cell culture laboratory.
5   Explains cloning and transfection techniques
6   Describes and compares cell characterization and separation techniques
7   Prepares culture media; seeds cells in culture; maintains cells in culture; passes/splits suspension and monolayer cultures; freezes and thaws cells; performs viability tests and transfection procedures.
8   Monitors culture cells daily and keeps his/her laboratory notebook accordingly.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Introduction The biology of culture cells Instruments used in the cell culture laboratory
2 Instruments consumables and media used in cell culture techniques
3 Feature of the cell culture media Preparation of steril Bss & media Sterilization procedures, aseptic tehnique and bio-safety in the cell culture media
4 Suspension culture maintenance and application of aseptic techniques Cell count and viability Different types of primary culture cells and cell lines
5 Monolayer culture splitting and maintenance
6 Recognizing contamination Cell banking and freezing Preperation of freezing media and freezing suspension culture cells
7 transformation and transfection CaPO3 transfection of GFP-vector (HeLa cells) Thawing and seeding frozen cells Preperation of freezing media and freezing suspension culture cells
8 Cell cloning, separation and selection methods Cell separation techniques and FACS Florescent microscoby or transfected cells Chracterization and differentiation of cells in culture
9 Midtermn exam
10 Project work on HeLa cell line (Discussion and application)
11 Project work on HeLa cell line (Discussion and application)
12 Project work on HeLa cell line (Discussion and application)
13 Project work on HeLa cell line (Discussion and application)
14 Evaluation and submision project works

Recomended or Required Reading

1- Freshney RI: "Culture of Animal Cells, Amanual of Basic Technique" Fourth ed., Wiley-Liss, 2000
2- Basic Cell Culture Protocols, 2nd ed.: Methods in Molecular Biology, Vol. 75 J.W. Pollard and J.M. Walker, ed., Humana Press, US, 1997
3- Animal Cell Biotechnology: Methods And Protocols N. Jenkins, ed., Humana Press, US, 1999
4- Cell and Tissue Culture for Medical Research A. Doyle and J.B. Griffiths, ed., John Wiley & Sons, Ltd, 2000
Supplementary Book(s):
References:
Materials:

Planned Learning Activities and Teaching Methods

Lectures: Basic theoretical concepts will be explained during lectures
Homework: Students are expected to hand in 4 written reports on topics that are given by the course coordinator.
Project: Each student will monitor, split, maintain, freeze, thaw and perform viability tests on a Hela cells given by the course coordinator during the course. Every observation and application is expected to be recorded in his/her laboratory notebook; which will be handed in at the end of the course.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 FIN FINAL EXAM
3 FCG FINAL COURSE GRADE MTE * 0.50 + FIN* 0.50
4 RST RESIT
5 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.50 + RST* 0.50


Further Notes About Assessment Methods

Learning objectives 1-6 will be assessed by a written mid-term exam and by the evaluation of written reports handed in as homework.
Learning objectives 7 and 8 will be assessed by the evaluation of the laboratory notebook handed in by the student; as well as direct observation of his/her lab work. Attendance will also be taken into consideration.
Grading (%):
Mid-term exam: 40%
Homework Assignments/Presentation: 10%
Projects & Laboratory work: 50 %

Assessment Criteria

To be announced.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

Tel: 412 4608 E-posta: zeynep.sercan@deu.edu.tr

Office Hours

Wednesday s 15:30-17:00

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 14 2 28
Tutorials 14 4 56
Preparing presentations 12 4 48
Lab Preparation 14 4 56
Preparation for final exam 1 10 10
Preparation for midterm exam 1 7 7
Preparing assignments 12 3 36
Preparations before/after weekly lectures 14 4 56
Midterm 1 3 3
Project presentation 1 3 3
TOTAL WORKLOAD (hours) 303

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11PO.12PO.13
LO.154
LO.254
LO.354
LO.45
LO.544
LO.6555
LO.75
LO.84445

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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