COURSE UNIT TITLE

: BIOELECTROCHEMISTRY

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
KIM 5123 BIOELECTROCHEMISTRY ELECTIVE 3 0 0 6

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR DOCTOR ŞENOL ALPAT

Offered to

M.Sc.Biochemistry
Chemistry
Chemistry
Ph.D in Biochemistry

Course Objective

In the scope of this lesson, the goal intended to be attained is to observe the manner of the electrochemical biomolecules through the use of the electro-analytical methods, and to give the general knowledge of the methods subjacent for the running principle of the biosensors.

Learning Outcomes of the Course Unit

1   Be able to interpret various electrochemical results of biological systems,
2   Be able to apply electrochemical methods to solve biohemistry and various biosensors problems
3   To have a comprehensive understanding of analytical chemistry application of electrochemistry and learn how electrochemistry is used in biosensors for ultrasensitive detection
4   Experimental design and implement
5   Be able to follow and interpret the relevant literature in this area

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 1.1. Basic principles of bioelectrochemistry
2 Electrodes and electrolytes in bioelectrochemistry 2.1. Working Electrodes 2.2. Referances Electrodes 2.3.Electrolytes
3 3.1. Use of voltammetric techniques in bioelectrochemistry
4 4.1. Cyclic voltammetry of biomolecules clarification of the mechanisms of electrochemical
5 Preparation of modified electrodes 5.1. Modified Electrodes 5.2. Carbon paste electrodes 5.3. Preparation of electrode with sol -gel technique 5.4. Preparation of electrode with nanoparticules 5.5. Preparation of electrode with SWCNT and MWCNT
6 Electrochemistry of redox proteins 6.1. protein electrochemistry 6.2. The nature of the electrode surface 6.3. the electrode surfaces of the SEM and AFM 6.4. Protein-protein complexes
7 Direct electrochemistry of enzyme 7.1. Thiol / disulfide exchange and redox proteins potentials 7.2. Electrochemistry of Fe-S proteins
8 8.1. Nanomaterial-based enzyme sensors
9 Membranes Bioelectrochemistry 9.1. Membranes potentials 9.2. Lipids 9.3. Na + / K +-ATP's activation of the electro 9.4. Lipid Later electrical conductivity 9.5. Cell membranes electrical conductivity
10 Electrochemistry of Nucleic acids 10.1. Polography of DNA 10.2. The nucleic acid components of the electrochemical properties 10.3. Electrochemistry of nucleic acids
11 Electrochemistry of neurotransmitters 11.1. electrochemistry of catechol 11.2. Dopamine, dopa, and serotonin electrochemistry 11.3. Epinephrine, norepinephrine, Electrochemistry
12 Electrochemistry and regeneration of coenzymes 12.1. Electrochemistry of coenzyme NADH 12.2. Electrochemistry of coenzyme NADPH 12.3. Electrochemical regeneration of coenzymes Presentations,homework
13 Electrochemistry and regeneration of coenzymes 13.1. Electrochemistry of coenzyme NADH 13.2. Electrochemistry of coenzyme NADPH 13.3. Electrochemical regeneration of coenzymes
14 Electrochemistry of Some important metabolites 14.1. Glutamate, lactate, acetyl choline, uric acid, Ascorbic Acid, Cholesterol and Glucose Presentations,homework

Recomended or Required Reading

Textbook(s): Lenaz. G., Milazzo G., (Ed), (1997), Bioelectrochemistry of Biomacromolecules. Basel, Birhauser Verlag, 385 p.
Supplementary Book(s): Brabec. V., Walz. D., Milazzo G., (Ed),(1996), Experimental Techniques in Bioelectrochemistry. Basel, Birhauser Verlag,527 p.
Walz. D., Milazzo G., Teissie J., (Ed), (2004),Bioelectrochemistry of Membranes. Basel, Birhauser Verlag, 527 p.
Scholz.F.,(Ed), (2002), Electroanalytical Methods. Berlin-Springer, 319 p.

Planned Learning Activities and Teaching Methods

1. Presentation
2. Interactive narrative
3. Question-answer technique
4. Home work

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 FIN FINAL EXAM
4 FCG FINAL COURSE GRADE MTE * 0.25 +ASG * 0.35 +FIN * 0.40
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.25 +ASG * 0.35 + RST * 0.40


Further Notes About Assessment Methods

None

Assessment Criteria

Meaningful learning of the basic concepts given in presentations, association of concepts with each other, establishing the cause-result relationships and making comments by using the information available for problems and evaluating idea generation are carried out with mid-term and final exams, active participation to the lecture, homework.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

senol.alpat@deu.edu.tr

Office Hours

Wednesday 13.30-15.30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 14 3 42
Preparations before/after weekly lectures 14 3 42
Preparation for midterm exam 1 10 10
Preparation for final exam 1 20 20
Preparing assignments 2 10 20
Preparing presentations 2 10 20
Final 1 2 2
Midterm 1 2 2
TOTAL WORKLOAD (hours) 158

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11
LO.155454455
LO.255555555
LO.3555555555
LO.45555
LO.55555

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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