Dokuz Eylül University Information Package / Courses Catalog

Description of Individual Course Units

Course Unit Code	Course Unit Title	Type Of Course	D	U	L	ECTS
KIM 5125	ELECTROPHORESIS AND ITS APPLICATIONS	ELECTIVE	3	0	0	6

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR DOCTOR RAZIYE ÖZTÜRK ÜREK

Offered to

M.Sc.Biochemistry
Chemistry
Chemistry
Ph.D in Biochemistry

Course Objective

In the concept of this course, the students will learn to fundamentals of electrophoretic methods that used to separate, identify and quantify biomolecules in medicine especially biochemistry and aimed to be known importance of samples in different fields.

Learning Outcomes of the Course Unit

1	know principles of electrophoresis and discuss to application fields,
2	know principles of isoelectric focusing and comprehend the importance,
3	explain to principles of polyacrylamide gel electrophoresis and discuss to application fields,
4	explain two-dimensional (2D) gel electrophoresis and discuss to applications in biochemistry and medicine,
5	have knowledge about applications of immunodiffusion and immunoelectrophoresis and give examples on the applications in medicine.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week	Subject	Description
1	Basic Theories and Principles of Electrophoresis
2	Basic Princiles of Isoelectric Focusing
3	Basic of Polyacrylamide Gel Electrophoresis
4	Applications and Strategies for Use of Polyacrylamide Electrophoresis
5	Sample Preparation for Electrophoresis, Aplications and Gel Dye
6	Analysis of Electrophoresis Gels
7	Agarose and Composite Polyacrylamide-Agarose Gel Electrophoresis
8	Agarose and Composite Polyacrylamide-Agarose Gel Electrophoresis and Analysis Nucleic Acid
9	Isoelectric focusing
10	Two-dimensional (2D) gel electrophoresis
11	Immunodiffusion
12	Immunoelectrophoresis
13	Isotakphoresis (Displacement Electrophoresis)
14	Student presentations

Recomended or Required Reading

Textbook(s): Michov B., Electrophoresis Fundamentals: Essential Theory and Practice, Walter de Gruyter GmbH & Co KG, 2022.
Dunbar B.S, Two-dimensional Electrophoresis and Immunological Techniques, Plenum Press, 1990, ISBN: 0-306-42839-3.
Supplementary Book(s): Hames B.D., Rickwood D, Gel Electrophoresis of Proteins, 1994, Oxford University Press, ISSN: 019 963074 7.

Planned Learning Activities and Teaching Methods

Presentation,
Questions and answers,
Homework

Assessment Methods

SORTING NUMBER	SHORT CODE	LONG CODE	FORMULA
1	ASG	ASSIGNMENT
2	MTE	MIDTERM EXAM
3	FIN	FINAL EXAM
4	FCG	FINAL COURSE GRADE	ASG * 0.30 + MTE * 0.30 + FIN * 0.40
5	RST	RESIT
6	FCGR	FINAL COURSE GRADE (RESIT)	ASG * 0.30 + MTE * 0.30 + RST * 0.40

Further Notes About Assessment Methods

None

Assessment Criteria

Meaningful learning of the basic concepts given in presentations, association of concepts with each other, establishing the cause-result relationships and making comments by using the information available for problems and evaluating idea generation are carried out with mid-term and final exams, active participation to the lecture, homework.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

Dokuz Eylul University, Faculty of Science, Department of Chemistry e-mail: raziye.urek@deu.edu.tr

Office Hours

Wednesday 15.00-16.00

Work Placement(s)

None

Workload Calculation

Activities	Number	Time (hours)	Total Work Load (hours)
Lectures	14	3	42
Preparations before/after weekly lectures	14	3	42
Preparation for midterm exam	1	20	20
Preparation for final exam	1	25	25
Preparing assignments	1	18	18
Final	1	2	2
Midterm	1	1	1
TOTAL WORKLOAD (hours)			150

Contribution of Learning Outcomes to Programme Outcomes

PO/LO	PO.1	PO.2	PO.3	PO.4	PO.5	PO.6	PO.7	PO.8	PO.9	PO.10	PO.11
LO.1	4	4	4	5	2	4	3	4	4	5	4
LO.2	4	4	4	5	2	4	3	4	4	5	4
LO.3	4	4	4	5	2	4	3	5	5	5	5
LO.4	4	4	4	5	2	5	5	5	5	5	5
LO.5	4	5	4	5	2	5	5	5	5	5	5

COURSE UNIT TITLE

DEGREE PROGRAMMES

Description of Individual Course Units

Offered By

Level of Course Unit

Course Coordinator

Offered to

Course Objective

Learning Outcomes of the Course Unit

Mode of Delivery

Prerequisites and Co-requisites

Recomended Optional Programme Components

Course Contents

Recomended or Required Reading

Planned Learning Activities and Teaching Methods

Assessment Methods

Further Notes About Assessment Methods

Assessment Criteria

Language of Instruction

Course Policies and Rules

Contact Details for the Lecturer(s)

Office Hours

Work Placement(s)

Workload Calculation

Contribution of Learning Outcomes to Programme Outcomes

CONTACT INFORMATION