Dokuz Eylül University Information Package / Courses Catalog

Description of Individual Course Units

Course Unit Code	Course Unit Title	Type Of Course	D	U	L	ECTS
KIM 5113	PROTEIN PURIFICATION METHODS	ELECTIVE	3	0	0	8

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR DOCTOR RAZIYE ÖZTÜRK ÜREK

Offered to

M.Sc.Biochemistry
Chemistry
Chemistry
Ph.D in Biochemistry

Course Objective

It is obligatory that proteins which are one of the macromolecules playing important roles in the life of alives are purified for the applications in many fields such as medical, nutrient and pharmacology. Methods, techniques and strategies utilised for the purification of proteins will be discussed within the context of this course.

Learning Outcomes of the Course Unit

1	Have a knowledge about protein purification methods,
2	Have knowledge about structure of proteins and amino acids so determine which method is proper for purification of proteins,
3	Interpret the possible problems during the purification process,
4	Explain the application fields of protein purification,
5	Examine high throughput screening techniques in protein purification.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week	Subject	Description
1	Introduction to Protein Purification
2	Gel Filtration: Size Exclusion Chromatography, Ion Exchange Chromatography
3	High Resolution Reversed-Phase Chromatography
4	Hydrophobic Interaction Chromatography
5	Immobilized Metal Ion Chromatography
6	Affinity Chromatography
7	Other Separation Methods and Related Techniques: Membran Separations
8	Other Separation Methods and Related Techniques 2
9	Gel Electrophoresis, Conventional Isoelectric Focusing
10	Two Dimensional Electrophoresis in Proteomics
11	Protein Elution and Blotting Techniques
12	Capillary Electrophoresis
13	Separation Method Optimization: High Throughput Screening Techniques in Protein Purification
14	Student Presentations

Recomended or Required Reading

Textbook(s): Bonner P., Protein Pur,f,cation. Taylor & Francis, 2018.
Janson J. C., Protein Purification: Principles, High Resolution Methods, and Applications, Wiley-VCH, 1998.

Planned Learning Activities and Teaching Methods

Presentation
Question-answer technique
Homework

Assessment Methods

SORTING NUMBER	SHORT CODE	LONG CODE	FORMULA
1	MTE	MIDTERM EXAM
2	ASG	ASSIGNMENT
3	FIN	FINAL EXAM
4	FCG	FINAL COURSE GRADE	MTE 0.35 + ASG 0.25 +FIN *0.40
5	RST	RESIT
6	FCGR	FINAL COURSE GRADE (RESIT)	MTE 0.35 + ASG 0.25 +RST *0.40

Further Notes About Assessment Methods

None

Assessment Criteria

Meaningful learning of the basic concepts given in presentations, association of concepts with each other, establishing the cause-result relationships and making comments by using the information available for problems and evaluating idea generation are carried out with mid-term, final and resit exams, active participation to the lecture, homework.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

Dokuz Eylul University, Faculty of Science, Department of Chemistry
e-mail: raziye.urek@deu.edu.tr

Office Hours

Wednesday 13.30-14.30

Work Placement(s)

None

Workload Calculation

Activities	Number	Time (hours)	Total Work Load (hours)
Lectures	14	3	42
Preparations before/after weekly lectures	14	3	42
Preparation for midterm exam	1	35	35
Preparation for final exam	1	45	45
Preparing presentations	1	25	25
Final	1	2	2
Midterm	1	1	1
TOTAL WORKLOAD (hours)			192

Contribution of Learning Outcomes to Programme Outcomes

PO/LO	PO.1	PO.2	PO.3	PO.4	PO.6	PO.8	PO.9	PO.10	PO.11
LO.1	5	5	5	5	5	5	5
LO.2	5	5	5	5	5	5	5		5
LO.3	5	5	5	5	5	5	5
LO.4	5	5	5	5	5	5	5
LO.5		5	5	5	5	5	5	5	5

COURSE UNIT TITLE

DEGREE PROGRAMMES

Description of Individual Course Units

Offered By

Level of Course Unit

Course Coordinator

Offered to

Course Objective

Learning Outcomes of the Course Unit

Mode of Delivery

Prerequisites and Co-requisites

Recomended Optional Programme Components

Course Contents

Recomended or Required Reading

Planned Learning Activities and Teaching Methods

Assessment Methods

Further Notes About Assessment Methods

Assessment Criteria

Language of Instruction

Course Policies and Rules

Contact Details for the Lecturer(s)

Office Hours

Work Placement(s)

Workload Calculation

Contribution of Learning Outcomes to Programme Outcomes

CONTACT INFORMATION