COURSE UNIT TITLE

: HISTOLOGICAL PREPARATION TECHNIQUES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
BYL 5025 HISTOLOGICAL PREPARATION TECHNIQUES ELECTIVE 2 2 0 6

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

ASSOCIATE PROFESSOR ELIF YILDIRIM CAYNAK

Offered to

Ph.D. in Biology
Biology

Course Objective

Learn how to assess histological sections under light microscope and follow the stages of preparation and staining of histological sections.

Learning Outcomes of the Course Unit

1   To obtain tissue sections using microtome.
2   To produce solutions when faced with unforeseen situations in technical steps.
3   To improve hand skills.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Microscope types Introduction to light microscope The importance of preparing histological preparation
2 Living tissue supply Fixation and its purpose Fixative selection and preparation Factors adversely affecting the fixation
3 Dehydration and clearing
4 Embedding in paraffin Preparing tissue for sections
5 Introduction of microtome Sectioning
6 Sectioning
7 Points to be considered in staining Importance of staining Importance of appropriate stain selection
8 Overview
9 Preparation of stain Preparation stages
10 Staining techniques
11 Staining techniques
12 Staining techniques
13 Storage of sections Archiving sections
14 Examination of cross-sections under microscope
15 Final examination

Recomended or Required Reading

Junqueira Temel Histoloji Atlas Kitabı.

Planned Learning Activities and Teaching Methods

The course is taught in a lecture, class presentation and discussion format. All class members are expected to attend and both the lecture and take part in the discussion sessions.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 FIN FINAL EXAM
3 FCG FINAL COURSE GRADE MTE * 0.40 + FIN * 0.60
4 RST RESIT
5 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.40 + RST * 0.60


Further Notes About Assessment Methods

None

Assessment Criteria

Student will be evaluated with midterm exams, homework presentation and final exam.

Language of Instruction

Turkish

Course Policies and Rules

Attendance to at least 70% for the lectures is an essential requirement of this course and is the responsibility of the student. It is necessary that attendance to the lecture and homework delivery must be on time. Any unethical behavior that occurs either in presentations or in exams will be dealt with as outlined in school policy

Contact Details for the Lecturer(s)

yildirim.elif@deu.edu.tr

Office Hours

will be announced

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 14 5 70
Preparations before/after weekly lectures 14 2 28
Preparation for midterm exam 1 16 16
Preparation for final exam 1 16 16
Final 1 4 4
Midterm 1 4 4
TOTAL WORKLOAD (hours) 138

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11PO.12
LO.1554454544555
LO.2455554544545
LO.3545554554554