COURSE UNIT TITLE

: BASIC TECHNIQUES IN BIOCHEMISTRY

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
KIM 4249 BASIC TECHNIQUES IN BIOCHEMISTRY ELECTIVE 2 0 0 3

Offered By

Chemistry

Level of Course Unit

First Cycle Programmes (Bachelor's Degree)

Course Coordinator

PROFESSOR DOCTOR HÜLYA AYAR KAYALI

Offered to

Chemistry

Course Objective

In this lecture the students will learn Biological buffers and sample preparation techniques; Techniques for separating and purifying biomolecules; Spectroscopic analysis methods; Microscopy techniques and imaging methods; Chromatographic techniques; High-performance liquid chromatography and gas chromatography; Mass spectrometry and its applications in biochemistry; PCR and RT-PCR techniques; Electrophoresis applications and immunological techniques; Enzymatic analysis techniques, and Surface plasmon resonance technique.

Learning Outcomes of the Course Unit

1   At the end of this course, students able to have knowledge about the techniques used to prepare the sample for in biochemistry researches and generate idea,
2   In biochemistry studies can discuss the basis of pre-seperation methods to separate the target molecule from a sample, have knowledge about verifying the accuracy of further purification using analysis methods such as spectroscopy or chromatography.
3   Students have knowledge about microscopy imaging techniques used in biological research.
4   Students are informed about fundamental genetic or protein analyses that can be performed on a biologically derived sample and have expertise regarding applicability and parameters that require attention, and can evaluate them.
5   Students have knowledge about the principles that must be considered in enzyme activity measurements from biological sources and can make condition-dependent evaluations.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Biological Buffers and Sample Preparation Techniques
2 Techniques for Separation and Purification of Biomolecules
3 Biochemical Spectrophotometric and Fluorometric Assay Methods
4 Microscopy Techniques and Imaging Methods
5 Chromatography Types and Their Applications
6 High-Performance Liquid Chromatography and Gas Chromatography
7 Mass Spectrometry and Its Applications in Biochemistry
8 PCR and RT-PCR Techniques
9 Electrophoresis Applications and Immunological Techniques
10 Enzymatic Analysis Techniques and Surface Plasmon Resonance Technique
11 Discussion of Research Techniques Based on the Literature-1
12 Discussion of Research Techniques Based on the Literature-2
13 Discussion of Research Techniques Based on the Literature-3
14 Discussion of Research Techniques Based on the Literature-4

Recomended or Required Reading

Primary source: Glencross H, Ahmed N, Wang Q, Biomedical Science Practice, 978-0-19-8831228, Oxford University Press, 2023.
S.R. Mikkelsen and E. Corton, Bioanalytical Chemistry, Wiley Interscience, 2004.
Supplementary sources: K. Wilson, J. Walker, Principles and Techniques of Practical Biochemistry, ISBN: 0 521 65104 2, Cambridge University Press, 2000.
D. J. Holme, H. Peck, Analytical Biochemistry, Prentice Hall, 1998.

Planned Learning Activities and Teaching Methods

Presentation
Questions and answers
Homework

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 PAR PARTICIPATION
4 FIN FINAL EXAM
5 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.30 + ASG * 0.20 + PAR * 0.10 + FIN * 0.40
6 RST RESIT
7 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.30 + ASG * 0.20 + PAR * 0.10 + RST * 0.40


Further Notes About Assessment Methods

None

Assessment Criteria

The meaningful learning of the fundamental concepts presented in lectures, the ability to relate these concepts to one another, establish cause-and-effect relationships, and use existing knowledge to interpret and generate ideas for questions or problems will be assessed through a midterm exam, final exam, active participation in the course, and assignments.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

DEU Science Fac., Chemistry Dep. Biochemistry Division, Buca-IZMIR
e-mail: hulya.kayali@deu.edu.tr

Office Hours

Wednesday 16:00-17:00

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 14 2 28
Preparations before/after weekly lectures 14 1 14
Preparation for midterm exam 1 10 10
Preparation for final exam 1 15 15
Preparing assignments 1 5 5
Final 1 2 2
Midterm 1 1 1
TOTAL WORKLOAD (hours) 75

Contribution of Learning Outcomes to Programme Outcomes

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LO.3555555555
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CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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