COURSE UNIT TITLE

: SEPARATION PROCESS IN BIOTECHNOLOGY

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
BYT 5001 SEPARATION PROCESS IN BIOTECHNOLOGY ELECTIVE 3 0 0 8

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR DOCTOR LEMAN TARHAN

Offered to

Ph.D. in Biotechnology
Ph.D. in Biotechnology
BIOTECHNOLOGY

Course Objective

Various methods, techniques and strategies which can be applied for isolation and purification of particularly proteins and products obtained in biotechnology will be dealt, possible problems will be discussed, optimization criteria will be evaluated.

Learning Outcomes of the Course Unit

1   Have a knowledge about basic principle and strategies in biotechnological seperation applications and discuss,
2   Have a knowledge about cell disruption methods and produce alternatives,
3   Have a knowledge about microfiltration, ultrafiltration, concantration, purification, electrophoretic methods used in purification processes and their application areas,
4   Make applications of removal of salts and buffer exchange, dialysis, diafiltration, gel filtration,
5   Have a knowledge about applications of ion exchange, hydrophobic interaction and affinity chromatographies in bioprocesses, protein chromatography and produce an idea.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 General Principles and Strategies in Separation
2 Cell Lysis Techniques
3 Isolation of Membrane Proteins and Protein Aggregates
4 Centrifuge Through Preparation of Crude Extract
5 Microfiltration in Purification Processes
6 Ultrafiltration in Purification Processes
7 Removal of Salts and Buffer Exchange, Dialysis, Diafiltration, Gel Filtration
8 Mid-term Exam
9 Concentration and Purification in Purification Processes
10 Purification by Two-Phase Systems
11 Electrophoretic Methods
12 Ion Exchange Chromatography; Hydrophobic Interaction Chromatography and The Applications in Bioprocesses
13 Fast Chromatography; Affinity Chromatography and The Applications in Bioprocesses, New Approaches in Protein Chromatography
14 Homework and Presentations

Recomended or Required Reading

Textbook: Hermanson, G. T., Mallia A. K. & Smith, P. K., Immobilized Affinity Ligand Techniques, Academic Press, 1992.
References: Kirschning, A. & Bannwarth, W., Immobilized Catalysts: Solid Phases, Immobilization and Applications, Springer, 2004.

Planned Learning Activities and Teaching Methods

1. Presentation
2. Question-answer technique
3. Homework

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 PRS PRESENTATION
4 FIN FINAL EXAM
5 FCG FINAL COURSE GRADE MTE* 0.30 + ASG * 0.20 + PRS * 0.10 + FIN * 0.40
6 RST RESIT
7 FCGR FINAL COURSE GRADE (RESIT) MTE* 0.30 + ASG * 0.20 + PRS * 0.10 + RST * 0.40


*** Resit Exam is Not Administered in Institutions Where Resit is not Applicable.

Further Notes About Assessment Methods

None

Assessment Criteria

Meaningful learning of the basic concepts given in presentations, association of concepts with each other, establishing the cause-result relationships and making comments by using the information available for problems and evaluating idea generation are carried out with mid-term, final and resit exams homework and presentations.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

Dokuz Eylul University, Science Faculty, Chemistry Department
leman.tarhan@hotmail.com

Office Hours

Wednesday 13.30-15.30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 13 3 39
Preparation before/after weekly lectures 13 3 39
Preparing Homework and Presentations 1 35 35
Preparation for Final Exam 1 40 40
Preparation for Mid-term Exam 1 35 35
Final 1 2 2
Mid-term 1 2 2
TOTAL WORKLOAD (hours) 192

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6
LO.1545435
LO.2545435
LO.3545435
LO.4545435
LO.5545435