COURSE UNIT TITLE

: HPLC IN ANALYSIS OF BIOLOGICAL MOLECULES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
BYT 5005 HPLC IN ANALYSIS OF BIOLOGICAL MOLECULES ELECTIVE 3 0 0 8

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR DOCTOR HÜLYA AYAR KAYALI

Offered to

Ph.D. in Biotechnology
Ph.D. in Biotechnology
BIOTECHNOLOGY

Course Objective

HPLC is a chromatographic technique used to separate a mixture of compounds with the purpose of identifying,quantifying and purifying the individual components of the mixture.HPLC method has been used for qualitative and quantitative determination of drug,pesticide residues,hormone and vitamine levels as well as other metabolites in biological samples.The course aims to provide an introduction and applicabilty of HPLC,which is a technique for the separation and determination of organic and inorganic solutes in any samples especially biological,pharmaceutical,food,environmental,industrial.

Learning Outcomes of the Course Unit

1   Comprehend the basic principle of HPLC,
2   Realize the column, detecter used in HPLC and differences of these,
3   Study sample preparation techniques for HPLC,
4   Be carried out biochemical applications and develop a method in HPLC,
5   Realize other technique combinations as MS with HPLC.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Introduction to the HPLC
2 Principles and Terms of HPLC
3 Basics of Separations
4 Detection Sensitivity and Selectivity
5 Sample Preparation and Column
6 Non-ionic samples: Reversed and Normal Phase HPLC
7 Ionic samples: Reversed Phase, ion pair, ion exchanger HPLC
8 Midterm Exam
9 Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates and Related Compounds
10 Special Needs of Biochemical HPLC
11 Peptide and Protein Separation
12 Ion Exchanger HPLC
13 Oligonucleotide Separation
14 Size-Exclusion Chromatography

Recomended or Required Reading

Textbook(s): Snyder, L. R., Kirkland, J. J. & Glach, J. L. Practical HPLC Method Development A Wiley-Interscience Publication, 1997 .
Supplementary Book(s): Snyder, L. R., Kirkland, J.J. & Dolan, J. W. Introduction To Modern Liquid Chromatography
References:
Materials:

Planned Learning Activities and Teaching Methods

1. Presentation
2. Interactive narrative
3. Question-answer technique
4. Homework

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 PRS PRESENTATION
4 FIN FINAL EXAM
5 FCG FINAL COURSE GRADE MTE* 0.30 + ASG * 0.20 + PRS * 0.10 + FIN * 0.40
6 RST RESIT
7 FCGR FINAL COURSE GRADE (RESIT) MTE* 0.30 + ASG * 0.20 + PRS * 0.10 + RST * 0.40


*** Resit Exam is Not Administered in Institutions Where Resit is not Applicable.

Further Notes About Assessment Methods

None

Assessment Criteria

Meaningful learning of the basic concepts given in presentations, association of concepts with each other, establishing the cause-result relationships and making comments by using the information available for problems and evaluating idea generation are carried out with mid-term and final exams, active participation to the lecture, homework.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

Dokuz Eylül University, Science Faculty, Chemistry Department, Biochemistry Division, 35160, Buca, Izmir, Turkey
e-mail:hulya.kayali@deu.edu.tr
Tel: +90 232 3018690

Office Hours

Wednesday 13.30-15.30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 13 3 39
Preparation before/after weekly lectures 13 3 39
Preparation for Mid-term Exam 1 20 20
Preparation for Final Exam 1 25 25
Preparing Individual Assignments 3 10 30
Preparing Presentations 3 12 36
Final 1 2 2
Midterm 1 2 2
TOTAL WORKLOAD (hours) 193

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6
LO.1535345
LO.2535345
LO.3535345
LO.4545455
LO.5545455